Fibroblasts/Epithelial cells Antibody (D7-FIB)


Flow Cytometry: Fibroblasts/Epithelial cells Antibody (D7-FIB) [NB600-777] - Fibroblast Marker Staining of KG1 cells.

Product Details

Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC-Fr, IP
1.0 mg/ml

Fibroblasts/Epithelial cells Antibody (D7-FIB) Summary

Human foreskin fibroblasts
Cell Membrane
NB600-777 recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (3). This product is routinely tested in flow cytometry on the KG1 cell line.
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS. pH 7.4
0.09% Sodium Azide
1.0 mg/ml
Protein G purified


Application Notes
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. This antibody does not work in Immunohistochemistry-Paraffin. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27416040).
Read Publication using NB600-777.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibroblasts/Epithelial cells Antibody (NB600-777)(1)

We have publications tested in 1 application: ICC/IF.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB600-777AF405
Alexa Fluor (R) 488 Labeled NB600-777AF488
Alexa Fluor (R) 647 Labeled NB600-777AF647
Alexa Fluor (R) 700 Labeled NB600-777AF700
Allophycocyanin Labeled NB600-777APC
DyLight 350 Labeled NB600-777UV
DyLight 405 Labeled NB600-777V
DyLight 405LS Labeled NB600-777V3
DyLight 488 Labeled NB600-777G
DyLight 680 Labeled NB600-777FR
DyLight 755 Labeled NB600-777IR
PE Labeled NB600-777PE
PerCP Labeled NB600-777PCP

Additional Fibroblasts/Epithelial cells Products

Fibroblasts/Epithelial cells NB600-777

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