Fibroblasts/Epithelial cells Antibody (D7-FIB) [DyLight 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC-Fr
Clone
D7-FIB
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Fibroblasts/Epithelial cells Antibody (D7-FIB) [DyLight 488] Summary

Immunogen
Human foreskin fibroblasts
Localization
Cell Membrane
Specificity
NB600-777 recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (3). This product is routinely tested in flow cytometry on the KG1 cell line.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB600-777APC
DyLight 350 Labeled NB600-777UV
DyLight 405 Labeled NB600-777V
DyLight 680 Labeled NB600-777FR
DyLight 755 Labeled NB600-777IR
PE Labeled NB600-777PE
PerCP Labeled NB600-777PCP

Additional Fibroblasts/Epithelial cells Products

Fibroblasts/Epithelial cells NB600-777G

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