Feline Leukaemia Virus gp70 Antibody (C11D8) - BSA Free

• Reactivity: V-Vi
• Applications: WB, Flow, IHC, IP
• Clone: C11D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Feline Leukaemia Virus gp70 Antibody (C11D8) - BSA Free Summary

• Description: Novus Biologicals Mouse Feline Leukaemia Virus gp70 Antibody (C11D8) - BSA Free (NB100-65565) is a monoclonal antibody validated for use in IHC, WB, Flow and IP. Anti-Feline Leukaemia Virus gp70 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Made to Viral FELINE LEUKAEMIA VIRUS gp70
• Specificity: NB100-65565 recognizes the gp85/gp70 envelope glycoprotein of feline leukaemia virus. The antibody specifically recognizes the epitope defined by amino acids 214-218 of the gp70 sequence (aa MGPNL). Some weak cross-reactivity with the sequence MGPLL found in the FeLV p15e membrane protein may also occur. This antibody effectively neutralises all three FeLV subgroups (A,B and C).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Removal of sodium azide is recommended prior to use in functional assays.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: <0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Feline Leukaemia Virus gp70 Antibody (C11D8) - BSA Free

• Env polyprotein
• Feline Leukemia Virus glycoprotein 70
• Glycoprotein gp70 [Precursor]
• GP70 envelope protein
• gp70 protein (SU)
• p15E protein (TM)
• p15E protein

Background

FeLV is a retrovirus (family Oncovirinae) that can cause severe immunosuppression, severe non regenerative anemia, and a variety of neoplasms. The 3 main subtypes (FeLVA, FeLVB and FeLVC) may act independently or together to cause a variety of diseases. The major envelope glycoprotein (gp70) is used to bind to target cells and varies with the FeLV subtype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Feline Leukaemia Virus gp70 Antibody (NB100-65565)(1)

Publication using NB100-65565

• Roccabianca P, Turchetto S, Ferrari N et al. Feline lymphoplasmacytic rhinitis (FLPCR): Severity of inflammation correlates with reduced mucosal IgA expression Veterinary immunology and immunopathology 2021-02-05 [PMID: 33611160] (IHC-P, Virus)
  
  Details:
  Feline Leukaemia Virus gp70

Bioinformatics

• Uniprot:
  • Q85525(Virus)