FAM20B Antibody (1018512) [Unconjugated]

Western blot shows lysates of ZR-75 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FAM20B Monoclonal Antibody (Catalog # MAB8427) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FAM20B at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 1018512
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FAM20B Antibody (1018512) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human FAM20B
  Asn27-Leu409
  Accession # O75063
• Specificity: Detects human FAM20B in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FAM20B Antibody (1018512) [Unconjugated]

• EC 2.7.1.-
• FAM20B
• family with sequence similarity 20, member B
• Glycosaminoglycan Xylosylkinase
• gxk1
• KIAA0475
• Protein FAM20B
• XYLK
• Xylose Kinase

Background

FAM20B is a member of the FAM20 protein family that has three members in mammals (FAM20A, FAM20B, FAM20C) (1). Human FAM20B shares 97% amino acid sequence identity with mouse and rat FAM20B. FAM20B localizes to the Golgi apparatus where it phosphorylates the xylose residue in the glycosaminoglycan (GAG)-protein linkage region of proteoglycans, which leads to enhanced GAG biosynthesis (2). Accordingly, chondroitin sulfate and heparan sulfate production is increased when FAM20B is over-expressed and reduced following FAM20B knockdown (2). FAM20B knockout mice display embryonic lethality at day E13.5, suggesting that FAM20B has an important role during development (3). Furthermore, in zebrafish, FAM20B mutations result in reduced cartilage matrix production and skeletal defects (4). The enzymatic activity of recombinant human FAM20B is measured using a phosphatase-coupled method (5).

1. Nalbant, D. et al. (2005) BMC Genomics 6:11.
2. Koike, T. et al. (2009) Biochem. J. 421:157.
3. Vogel, P. et al. (2012) Vet. Pathol. 49:998.
4. Eames, B.F. et al. (2011) PLoS Genet. 7:e1002246.
5. Wu, Z.L. (2011) PLoS One 6:e23172.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FAM20B Antibody (MAB8427)(1)

Publication using MAB8427

• T Koike, T Mikami, JI Tamura, H Kitagawa Altered sulfation status of FAM20C-dependent chondroitin sulfate is associated with osteosclerotic bone dysplasia Nature Communications, 2022-12-26;13(1):7952. 2022-12-26 [PMID: 36572689] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • O75063()