Epithelial cell marker Antibody (LH39) - BSA Free

• Reactivity: Pm
• Applications: WB, IHC, IP
• Clone: LH39
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Epithelial cell marker Antibody (LH39) - BSA Free Summary

• Description: Novus Biologicals Mouse Epithelial cell marker Antibody (LH39) - BSA Free (NB100-2690) is a monoclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Cells from a single cell suspension of epidermal cells (obtained from fresh human neonatal foreskin) were lysed in Nonidet P40 in phosphate buffered saline and the insoluble pellet was sonicated to prepare insoluble fractions.
• Localization: Type I membrane protein. Two secreted forms are also produced.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: LH39 precipitates a polypeptide of 185 kDa from cultured keratinocytes. Positive control(s): Cultured keratinocytes.
• Theoretical MW: 185 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Background

MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. This antibody is potentially useful in the study of benign and malignant human vascular disorders, diseases and tumours. Associated with angiogenesis, epithelial neoplasms and conditions of tissue regeneration and repair such as wound healing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Entrez:
  • 4582(Human)