Eosinophil Antibody (BMK-13)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC, IHC-Fr, IHC-P
Clone
BMK-13
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Eosinophil Antibody (BMK-13) Summary

Immunogen
The antibody reacts with the Eosinophil Major Basic Protein antigen.
Specificity
BMK-13 binds to the 10 kD eosinophil Major Basic Protein (MBP) of both resting and activated eosinophils in cytospins and frozen sections of bronchial and skin biopsies of allergic sites and normal sites and thus can be used as a "pan-eosinophil" marker. The antibody BMK-13 stains in frozen sections of bronchial biopsies from atopic asthmatics, rhinitics and normal non-atopic subjects, substantially higher counts of positive cells when compared to EG1, EG2 and chromotrope 2R. BMK-13 cross-reacts weakly with human basophils, which also contain low level of this protein. It does not cross-react with any other human protein or cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This mAb is a very useful tool for clinical and experimental allergy studies. BMK-13 is a pan-eosinophil marker in both frozen sections and paraffin embedded tissues. It stains in bronchial sections 98% of the periphere blood eosinophils. Further, the antibody is most useful for immunohistochemistry on frozen sections is the APAAP method. BMK-13 also binds to eosinophils in paraffin-embedded tissue. A 1:30 dilution in PBS is recommended. Incubation time: 1 hour at RT. Enzymatic pretreatment is necessary. This antibody is suitable for blood smears (cytospins) if fixed with methanol-acetone mixture (1:1, v/v). It is unlikely, without permeabilization, that the BMK-13 is suitable for the flow cytometry because BMK-13 stains intracellular, stored proteins.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS + 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for Eosinophil Antibody (BMK-13)

  • BMPG
  • Bone marrow proteoglycan
  • EMBP
  • Eosinophil granule major basic protein
  • Eosinophil major basic protein
  • MBP
  • MGC14537
  • MPG
  • Natural killer cell activator
  • Pregnancy associated major basic protein
  • PRG 2
  • Proteoglycan 2 bone marrow
  • Proteoglycan 2 preproprotein
  • Proteoglycan 2
  • Proteoglycan2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Eosinophil Antibody (NBP1-42140). (Showing 1 - 1 of 1 FAQ).

  1. I would appreciate it very much if you could let me know the ingredients of the buffer this antibody is stored in?
    • This antibody is supplied as 1 ml purified antibody (conc. 100 ug/ml) in PBS + 0.1% BSA + 0.02% sodium azide.

Secondary Antibodies

 

Isotype Controls

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