![Western Blot: EosFP Antibody [NBP3-05557] - Western blot analysis of EosFP antibody. Lane 1: Non-transfected HEK293 crude homogenate. Lane 2: Crude homogenate of transfected HEK293 cells which overexpress protein EosFP. Lane S: Protein standard of indicated molecular weight RPCA-EosFP at 1:1,000 dilution reveals a strong clean band at ~25 kDa corresponding to EosFP in transfected cells, which is absent from non-transfected cells.](http://images.novusbio.com/fullsize/EosFP-Antibody-Western-Blot-NBP3-05557-img0002.jpg "Western Blot: EosFP Antibody [NBP3-05557] - Western blot analysis of EosFP antibody. Lane 1: Non-transfected HEK293 crude homogenate. Lane 2: Crude homogenate of transfected HEK293 cells which overexpress protein EosFP. Lane S: Protein standard of indicated molecular weight RPCA-EosFP at 1:1,000 dilution reveals a strong clean band at ~25 kDa corresponding to EosFP in transfected cells, which is absent from non-transfected cells.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Full length recombinant EosFP protein expressed in E. coli |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Supplied as serum |
| Preservative | No Preservative |
| Purity | Unpurified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: EosFP Antibody [NBP3-05557] - HEK293 cells were transfected EosFP (left image) and stained with RPCA-EosFP antibody followed by goat anti rabbit Alexa-594 conjugate (middle image). The right image shows the two images superimposed, clearly RPCA-EosFP staining closely matches EosFP expression.](http://images.novusbio.com/fullsize/EosFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05557-img0001.jpg "Immunocytochemistry/Immunofluorescence: EosFP Antibody [NBP3-05557] - HEK293 cells were transfected EosFP (left image) and stained with RPCA-EosFP antibody followed by goat anti rabbit Alexa-594 conjugate (middle image). The right image shows the two images superimposed, clearly RPCA-EosFP staining closely matches EosFP expression.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")