![ELISA: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below. Capture: Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) (5 ug/mL) Detection: Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-25452_rabbit-enterovirus-d68-vp1-mab-hl1997-azide-and-bsa-free-212024935132.jpg "ELISA: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below. Capture: Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) (5 ug/mL) Detection: Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) (1 ug/mL)")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ELISA, LFA |
| Clone | HL1997 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of Enterovirus D68 VP1 |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Lateral Flow Assay: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair. Capture: Enterovirus D68 VP1 antibody (NBP3-25452 [HL1997]) Detection: Enterovirus D68 VP1 antibody (NBP3-13564 [GT1843]) Samples (80 ng) : 1. Enterovirus D68 VP1 protein 2. Lysis buffer](http://images.novusbio.com/fullsize/nbp3-25452_rabbit-enterovirus-d68-vp1-mab-hl1997-azide-and-bsa-free-212024939185.jpg "Lateral Flow Assay: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair. Capture: Enterovirus D68 VP1 antibody (NBP3-25452 [HL1997]) Detection: Enterovirus D68 VP1 antibody (NBP3-13564 [GT1843]) Samples (80 ng) : 1. Enterovirus D68 VP1 protein 2. Lysis buffer")
![Western Blot: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Non-infected (-) and infected (+) RD whole cell extracts (20 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25452_rabbit-enterovirus-d68-vp1-mab-hl1997-azide-and-bsa-free-2120249422623.jpg "Western Blot: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Non-infected (-) and infected (+) RD whole cell extracts (20 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Non-infected (-) and infected (+) RD whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25452_rabbit-enterovirus-d68-vp1-mab-hl1997-azide-and-bsa-free-2120249383176.jpg "Western Blot: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Non-infected (-) and infected (+) RD whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below. Capture: Enterovirus D68 VP1 antibody [GT1843] (NBP3-13564) (5 ug/mL) Detection: Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-25452_rabbit-enterovirus-d68-vp1-mab-hl1997-azide-and-bsa-free-212024940845.jpg "ELISA: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below. Capture: Enterovirus D68 VP1 antibody [GT1843] (NBP3-13564) (5 ug/mL) Detection: Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) (1 ug/mL)")
![Lateral Flow Assay: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair. Capture: Enterovirus D68 VP1 antibody (NBP3-13564 [GT1843]) Detection: Enterovirus D68 VP1 antibody (NBP3-25452 [HL1997]) Samples (80 ng) : 1. Enterovirus D68 VP1 protein 2. Lysis buffer](http://images.novusbio.com/fullsize/nbp3-25452_rabbit-enterovirus-d68-vp1-mab-hl1997-azide-and-bsa-free-212024939122.jpg "Lateral Flow Assay: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair. Capture: Enterovirus D68 VP1 antibody (NBP3-13564 [GT1843]) Detection: Enterovirus D68 VP1 antibody (NBP3-25452 [HL1997]) Samples (80 ng) : 1. Enterovirus D68 VP1 protein 2. Lysis buffer")
![Enterovirus D68 VP1 antibody [HL1997] detects Enterovirus D68 VP1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:2000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25452_rabbit-enterovirus-d68-vp1-mab-hl1997-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2611202518441010.jpg "Enterovirus D68 VP1 antibody [HL1997] detects Enterovirus D68 VP1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:2000.Blue: Fluoroshield with DAPI .")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25452_rabbit-enterovirus-d68-vp1-mab-hl1997-azide-and-bsa-free-western-blot-26112025182364.jpg "Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")