![Western Blot: E-Tag Epitope Tag Antibody [NB600-527] - Analysis using the HRP conjugate of NB600-527. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/E-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-527-img0004.jpg "Western Blot: E-Tag Epitope Tag Antibody [NB600-527] - Analysis using the HRP conjugate of NB600-527. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit E-Tag Epitope Tag Antibody - BSA Free (NB600-527) is a polyclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-E-Tag Epitope Tag Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Rabbits were immunized with E-Tag (GAPVPYPDPLEPR) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA - Coating: 1:100-1:500. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB600-527 | Applications | Species |
|---|---|---|
| Grun CN, Jain R, Schniederberend M, Shoemaker CB et Al. Bacterial cell surface characterization by phage display coupled to high-throughput sequencing Nat Commun 2024-08-29 [PMID: 39209859] |
Secondary Antibodies |
Isotype Controls |
Research Areas for E-Tag Epitope Tag Antibody (NB600-527)Find related products by research area.
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![Western Blot: E-Tag Epitope Tag Antibody [NB600-527] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/E-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-527-img0002.jpg "Western Blot: E-Tag Epitope Tag Antibody [NB600-527] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")