Dityrosine Antibody (7D4) - BSA Free

Western Blot: Dityrosine Antibody (7D4) [NBP2-59360] - Western Blot analysis of Dityrosine-BSA Conjugate showing detection of 67 kDa Dityrosine protein using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 7D4 (NBP2-59360). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: 3,5-Dibromotyrosine-BSA. Lane 4: Dityrosine-BSA. Lane 5: Bromotyrosine-BSA. Lane 6: 7-ketocholesterol-BSA. Load: 1 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody (NBP2-59360) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Immunocytochemistry/Immunofluorescence: Dityrosine Antibody (7D4) [NBP2-59360] - Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Dityrosine Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

• Reactivity: All-NA
• Applications: WB, ELISA, ICC/IF
• Clone: 7D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Dityrosine Antibody (7D4) - BSA Free Summary

• Description: Novus Biologicals Mouse Dityrosine Antibody (7D4) - BSA Free (NBP2-59360) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Dityrosine Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
• Specificity: Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Dityrosine Antibody (7D4) - BSA Free

• Dityrosine;Dityrosine (DT);DT;DY

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dityrosine Antibody (NBP2-59360)(1)

Publication using NBP2-59360

• Ziegler K, Kunert AT, Reinmuth-Selzle K et al. Chemical modification of pro-inflammatory proteins by peroxynitrite increases activation of TLR4 and NF-kappa B: Implications for the health effects of air pollution and oxidative stress Redox Biol 2020-07-20 [PMID: 32739154] (WB)