Dengue Virus 2 NS1 Antibody (969822) [Unconjugated]

Recombinant Dengue Virus 2 NS1 protein was seriallydiluted 2-fold and captured by Mouse Anti-Dengue Virus 2 MonoclonalAntibody (Catalog # MAB94392) coated on a Clear Polystyrene Microplate (Catalog #DY990).Mouse Anti-Dengue Virus 2 NS1 Monoclonal Antibody (Catalog #MAB94393)was biotinylated and incubated with the protein captured on the plate.Detection of the standard curve was achieved by incubating Streptavidin-HRP(Catalog # DY998)followed by Substrate Solution (Catalog # DY999)and stopping the enzymatic reaction with Stop Solution (Catalog #DY994).

• Reactivity: V
• Applications: ELISA
• Clone: 969822
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Dengue Virus 2 NS1 Antibody (969822) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant Dengue Virus 2 NS1 protein
  Asp1-Ala352
  Accession # NP_739584
• Specificity: Detects recombinant Dengue Virus 2 NS1 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dengue Virus 2 NS1 Antibody (969822) [Unconjugated]

• DEN-2
• Dengue NS1 protein
• Dengue NS1
• Dengue Virus 2 NS1
• Dengue virus Serotype 2
• Dengue virus Type 2
• Dengue Virus
• DENV2
• DENV2-NS1

Background

Dengue virus (DENV), the cause of Dengue fever, is a single positive-stranded RNA virus of the family Flaviviridae, genus Flavivirus. 5 closely related but antigenically distinct serotypes of the virus have been found: DENV-1, DENV-2, DENV-3, DENV-4 and DENV-5.  DENV has a genome of about 11,000 bases, encoding a single large polyprotein that is subsequently cleaved into several structural and non-structural peptides. The polyprotein is divided into three structural proteins, C, prM, E, seven non-structural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5, and short non-coding regions on both the 5' and 3' ends. DENV nonstructural protein-1 (NS1) is a secreted glycoprotein that is absent from viral particles but accumulates in the supernatant and on the plasma membrane of cells during infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • NP_739584()