Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free

Immunohistochemistry-Paraffin: Dendritic Cell Marker Antibody (AP-MAB0801) [NB110-85474] - Formalin fixed and paraffin embedded mouse spleen tissue section was subjected to IHC staining of mouse DCs using NB110-85474 (x 40).

Immunohistochemistry-Paraffin: Dendritic Cell Marker Antibody (AP-MAB0801) [NB110-85474] - 4% PFA fixed and paraffin embedded mouse Liver and Lung sections from LPS treated mice was subjected to staining (DAB) of DC marker.

Immunohistochemistry-Paraffin: Dendritic Cell Marker Antibody (AP-MAB0801) [NB110-85474] - Formalin fixed and paraffin embedded mouse spleen tissue section was subjected to IHC staining of mouse DCs using NB110-85474 (x 20).

• Reactivity: Mu
• Applications: Flow, IHC, CyTOF-ready
• Clone: AP-MAB0801
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free Summary

• Description: Novus Biologicals Rat Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free (NB110-85474) is a monoclonal antibody validated for use in IHC and Flow. Anti-Dendritic Cell Marker Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Mouse Dendritic cells
• Marker: Dendritic Cell Marker
• Specificity: Reacts with mouse dendritic cells.
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:25-1:50
• Application Notes: The enzymatic antigen retrieval method (proteinase K digestion) was used in IHC-P that led to the generation of the images posted on this site. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free

• DCs
• Dendritic cell

Background

Dendritic cells are potent antigen presenting cells that possess the ability to stimulate naive T cells. They are critical for the induction of T cell responses resulting in cell mediated immunity. Within different tissues, dendritic cells differentiate and become active in the taking up and processing of antigens, and their subsequent presentation on the cell surface linked to major histocompatibility (MHC) molecules. Upon appropriate stimulation, dendritic cells undergo further maturation and migrate to secondary lymphoid tissues where they present Ag to T cells and induce an immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dendritic Cell Marker Antibody (NB110-85474)(1)

Publication using NB110-85474

• Rasaei R, Kim E, Kim JY et al. Regulation of JAM2 Expression in the Lungs of Streptozotocin-Induced Diabetic Mice and Human Pluripotent Stem Cell-Derived Alveolar Organoids Biomedicines 2020-09-11 [PMID: 32932992] (IHC-P, Mouse)

FAQs for Dendritic Cell Marker Antibody (NB110-85474). (Showing 1 - 4 of 4 FAQ).

1. I have a question regarding the antibody AP-MAB0801: The product description is very unspecific, saying it recognizes a surface marker of mouse DCs. We would like to know which surface marker this antibody recognizes, so we know which subsets of DCs will be stained.
   • This clone detects the Dendritic Cell Inhibitory Receptor 2 on mouse DC cells.
2. Does the antibody stain DCs in mouse spleen sections?
   • Yes
3. Which DCs does it stain?
   • NB110-85474 will stain all mouse dendridic cells.
4. Could you let us know which kind of dendritic cells from mouse have been used to generate the antibody? We want to use the antibody for staining of intravascular dendritische cells of the central nervous system.
   • The lab has informed me that purified Spleen dendritic cells were used as immunogen for this clone. The lab also informed me that the immunogen cells were DC enriched cells from spleen cell suspension and for antibody binding 100% pure cells are not required.