DDX55 Antibody - BSA Free

Western Blot: DDX55 Antibody [NBP2-47490] - Analysis in human cell line NB4.

Immunocytochemistry/Immunofluorescence: DDX55 Antibody [NBP2-47490] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.

Immunohistochemistry-Paraffin: DDX55 Antibody [NBP2-47490] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: DDX55 Antibody [NBP2-47490] - Staining of human fallopian tube shows moderate nuclear and cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: DDX55 Antibody [NBP2-47490] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: DDX55 Antibody [NBP2-47490] - Staining of human stomach shows strong nuclear positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DDX55 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DDX55 Antibody - BSA Free (NBP2-47490) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: KMNEKRKREEGSDIEDEDMEELLNDTRLLKKLKKGKITEEEFEKGLLTTGKRTIKTVDLGISDLEDDC
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX55
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  DDX55 Protein (NBP2-47490PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for DDX55 Antibody - BSA Free

• DEAD (Asp-Glu-Ala-Asp) box polypeptide 55
• DEAD box protein 55
• EC 3.6.1
• EC 3.6.4.13
• FLJ16577
• KIAA1595ATP-dependent RNA helicase DDX55
• MGC33209

Background

DDX55 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX55