DDX24 Antibody

Western Blot: DDX24 Antibody [NB100-2224] - Detection of Human DDX24 on HeLa whole cell lysate using NB100-2224. DDX24 was also immunoprecipitated by rabbit anti-DDX24 antibody NB100-2225 and NB100-2226.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DDX24 Antibody Summary

• Description: Novus Biologicals Rabbit DDX24 Antibody (NB100-2224) is a polyclonal antibody validated for use in WB and IP. Anti-DDX24 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 24 using the numbering given in entry NP_065147.1 (GeneID 57062).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX24
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Control:

  DDX24 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX24 Antibody

• ATP-dependent RNA helicase DDX24
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
• DEAD box protein 24
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 24
• EC 3.6.1
• EC 3.6.4.13
• S. cerevisiae CHL1-like helicase

Background

DDX24 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. The specific function of DDX24 has not been elucidated. Alternate names for DDX24 include ATP-dependent RNA helicase DDX24, and DEAD box protein 24.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX24 Antibody (NB100-2224)(1)

Publication using NB100-2224

• Yamauchi T, Nishiyama M, Moroishi T et al. MDM2 Mediates Nonproteolytic Polyubiquitylation of the DEAD-Box RNA Helicase DDX24. Mol. Cell. Biol. 2014-06-30 [PMID: 24980433] (WB, Human)
  
  Details:
  Antibody used for WB of DDX24 in HCT116 cells

Bioinformatics

• Gene Symbol: DDX24
• Entrez:
  • 57062(Human)
• Uniprot:
  • NP_065147.1(Human)