Cytochrome P450 307a1 Antibody

• Reactivity: Dr
• Applications: WB, DB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.60 mg/ml

Cytochrome P450 307a1 Antibody Summary

• Description: Novus Biologicals Rabbit Cytochrome P450 307a1 Antibody (NBP1-78150) is a polyclonal antibody validated for use in WB and ELISA. Anti-Cytochrome P450 307a1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide taken form the Spook protein form within the C-terminal region (amino acid 500-543), the peptide was post-synthetically modified and conjugated to carrier protein to achieve desired antigenicity. The Spook peptide sequence was conserved in rat, mouse and human protein and also present in other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:10000
  • ELISA 1:10000
  • Western Blot 1:500
• Application Notes: NBP1-78150 is ideal for WB and ELISA applications, other applications have not been tested. This antibody does not cross react to other fly proteins. The species cross reactivity for this antibody is not examined fully.

Reactivity Notes

NBP1-78150 recognizes Drosophila Cytochrome P450 307a1

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
• Preservative: 0.02% Sodium Azide
• Concentration: 0.60 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cytochrome P450 307a1 Antibody

• 307a1
• CG10594
• Cyp307a1
• CYP307A1-Dm
• Dmspo
• spo spook
• Spo

Background

Transposable elements are found in the genomes of nearly all eukaryotes. The recent completion of the Release 3 euchromatic genomic sequence of Drosophila melanogaster by the Berkeley Drosophila Genome Project has provided precise sequence for the repetitive elements in the Drosophila euchromatin. The genomic sequence to describe the euchromatic transposable elements in the sequenced strain of this species identified several new genes. Out of 85 unknown and 8 novel family of transposable elements varying in copy number form 1-146 have been identified. A total of 1500 full and partial transposable elements were identified that make up about 4% of the fruit fly genome. Most of these transposable elements reside outside genes (1). Spook or cytochrome P450a1 enzyme are involved in the detoxification of alkaloids and in xenobiotics resistance in insects. Cytochrome P450s form a large and diverse family of heme-containing proteins capable of carrying out many different enzymatic reactions. Some P450 proteins carry out reaction that are essential for process such as hormone synthesis, while others are P450 enzymes are involved in detoxification of environmental compounds. Spook expression patterns in embryos and s stage of third instar larvae indicate the protein is in abundance in fat bodies, malpighian tubules and in distinct regions of the midgut, consistent with its role in detoxification (2). The biological functions also involve embryonic development via syncytical blastderm, ecdysone biosynthesis process, heat involution, cuticle development. Spook gene sequence location is at 3L:5583181..5585677 is a 543 aa (66kDa) protein. There are at least 14 alleles are reported for this gene. The D melanogaster Spook-selective antibodies were generated against unique antigenic sequence taken form the C-terminal region with in the amino acids 500-543. The sequence was conserved in several species of fruit flies, but was unique to spook/CYP301a1 protein and was not present in other proteins. The Spook antibodies were affinity purified over immobilized antigen based chromatography, and the affinity purified immunoglobulins are stabilized in antibody stabilization buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytochrome P450 307a1 Antibody (NBP1-78150)(1)

Publication using NBP1-78150

• Chung H, Sztal T, Pasricha S et al. Characterization of Drosophila melanogaster cytochrome P450 genes. Proc Natl Acad Sci U S A. 106(14): 5731-5736. 2009-04-07 [PMID: 19289821]

Bioinformatics

• Uniprot:
  • AAQ05973(Drosophila)