Cytochrome c Antibody (OTI4C7) - Azide and BSA Free

Images

 
Western Blot: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGC12965.
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C ...read more
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Adenocarcinoma of Human colon. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at ...read more
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C ...read more
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for ...read more
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Carcinoma of Human kidney tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Carcinoma of Human lung tissue. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C ...read more
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for ...read more
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 C for 3 min)
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Cytochrome c Antibody (OTI4C7) - Azide and BSA Free [NBP2-71949] - Analysis of Human tonsil within the normal limits using MGC12965 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI4C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Cytochrome c Antibody (OTI4C7) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human MGC12965(NP_940921) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYCS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunohistochemistry 1:150
Theoretical MW
34.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for Cytochrome c Antibody (OTI4C7) - Azide and BSA Free

  • CYCHCS
  • CYCS
  • Cytochrome c
  • cytochrome c, somatic
  • THC4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Cytochrome c Antibody (NBP2-71949) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-71949AF350
Alexa Fluor 405 NBP2-71949AF405
Alexa Fluor 488 NBP2-71949AF488
Alexa Fluor 532 NBP2-71949AF532
Alexa Fluor 594 NBP2-71949AF594
Alexa Fluor 647 NBP2-71949AF647
Alexa Fluor 700 NBP2-71949AF700
Alexa Fluor 750 NBP2-71949AF750
Biotin NBP2-71949B
DyLight 350 NBP2-71949UV
DyLight 405 NBP2-71949V
DyLight 488 NBP2-71949G
DyLight 550 NBP2-71949R
DyLight 594 NBP2-71949DL594
DyLight 650 NBP2-71949C
DyLight 680 NBP2-71949FR
DyLight 755 NBP2-71949IR
FITC NBP2-71949F
HRP NBP2-71949H
Janelia Fluor 549 NBP2-71949JF549
Janelia Fluor 646 NBP2-71949JF646

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Pathway Highlight: Three key factors that contribute to cellular heterogeneity in apoptosis
Have you ever wondered why cells in the same population respond differently to an apoptotic stimulus? Apoptosis, a form of programmed cell death, is vital for the removal of unwanted or damaged cells. As with most cellular processes, too much or to...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

Cytochrome C in Apoptosis, Immune Response and Cancer
Cytochrome C is an electron carrier protein that localizes in mitochondrion intermembrane space and has been identified as one of the key signaling molecules of apoptosis or programmed cell death. Suppression of the anti-apoptotic members or activatio...  Read full blog post.

Mitofilin and the Mitochondrial Inner Membrane Organizing System (MINOS)
Mitofilin was originally described as a heart muscle protein due to its high expression in the heart. Recently, analysis of the human heart mitochondrial proteome demonstrated that Mitofilin is one of the most abundant mitochondrial proteins (1). Rese...  Read full blog post.

Bax Research Gives New Insight into Oxidative Apoptosis
Bax is a member of the Bcl-2 family; an extensive range of proteins which play key roles in apoptosis, or programmed cell death, by regulating outer mitochondrial membrane permeability. We at Novus Biologicals are one of the leading antibody suppliers...  Read full blog post.

The Role of the Caspase 3 Antibody in Apoptosis Research
The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are...  Read full blog post.

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Bioinformatics

Gene Symbol CYCS