Clostridium Tetani Toxoid Antibody - BSA Free

Western Blot: Clostridium Tetani Toxoid Antibody [NBP1-78003] - Detection of a protein band at ~ 52 kDa corresponding to full length 6XHIS-TTFC fusion protein (arrowhead). Lanes 1-5 contain 10 ng, 5ng 2.5ng, 1.25 ng and 0.0625 ng protein respectively. MW markers are shown at the left for size comparison. After blocking, the membrane was probed with the primary antibody diluted to 1:100,000 followed by reaction with a 1:20,000 dilution of HRP conjugated donkey-anti-Rabbit IgG [H&L].

Western Blot: Clostridium Tetani Toxoid Antibody [NBP1-78003] - Shows detection of a protein band at 52 kDa corresponding to full length 6X HIS-TTFC fusion protein (arrowhead). Lane 1 shows no detection from non-specific antisera; Lane 2 shows detection of anti-TTFC. After blocking in 1% BSA, the membrane was probed with the primary antisera diluted to 1:1,000 in PBS followed by reaction with HRP conjugated Goat anti-Rabbit at 1:20,000 dilution. Picomax substrate was used to generate signal. Personal communication Lucia le Roux, MD Anderson Cancer Center.

Western blot using Anti-tetanus toxin C antibody shows detection of a protein band at ~ 52 kDa corresponding to full length 6XHIS-TTFC fusion protein. Lanes 1-5 contain 10ng, 5ng, 2.5ng, 1.25ng and 0.0625ng protein respectively. MW markers are shown at the left for size comparison. After blocking, the membrane was probed with the primary antibody diluted to 1:100,000 followed by reaction with a 1:20,000 dilution of HRP conjugated donkey-anti-Rabbit IgG [H&L]

• Reactivity: Ba
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Clostridium Tetani Toxoid Antibody - BSA Free Summary

• Description: The product was prepared from monospecific antiserum by delipidation and defibrination
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This Clostridium Tetani Toxoid Antibody was prepared from whole rabbit serum produced by repeated immunizations with a polyhistidine-tagged Clostridium Tetani Toxoid fusion protein expressed in E. coli corresponding to full length Clostridium Tetani Toxoid. (Uniprot: P04958)
• Specificity: This antibody is directed against the C fragment of Tetanus toxin derived from Clostridium tetani.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:50000-1:250000
  • Western Blot 1:1000-1:40000
• Application Notes: This antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 52 kDa in size corresponding to full-length TTFC. Specific conditions for reactivity should be optimized by the end user.

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with Clostridium tetani. Cross-reactivity with Clostridium Tetani Toxoid from other sources have not been determined

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Delipidation and Defibrination

Alternate Names for Clostridium Tetani Toxoid Antibody - BSA Free

• Clostridium tetani toxoid
• Tentoxylysin
• Tetanus toxin
• tetX

Background

Tetanus toxin C-fragment (TTc) is a non-toxic 52 kD polypeptide generated by papain cleavage of tetanus toxin, corresponding to the C-terminal 451 amino acids. The illness known as Tetanus is caused by a neurotoxin produced by the anaerobic bacterium Clostridium tetani. C. tetani is commonly found in soil, feces, and on many objects lying on the ground, such as rusty metal, hence puncture wounds are a common cause of tetanus. It acts upon the presynaptic membranes of both central and peripheral nervous systems to block the release of neurotransmitters. In its toxic form tetanus neurotoxin is a 150 kDa protein, consisting of two major components: a light chain and heavy chain. The light chain contains the enzymatic portion of the toxin and is responsible for its toxic effects. The heavy chain binds to the neuron and aids delivery of the light chain to the neuron interior. A single disulfide bond links light and heavy chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P04958(C. tetani)