Clostridium Botulinum Toxin A Antibody - Azide and BSA Free

• Reactivity: Ba
• Applications: WB, DB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Clostridium Botulinum Toxin A Antibody - Azide and BSA Free Summary

• Immunogen: Type A Neurotoxin
• Localization: Secreted
• Specificity: Clostridium botulinum Type A Neurotoxin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • ELISA 1:10000
  • Western Blot 1:100-1:2000
• Theoretical MW: 149 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

C. botulinum Type A Neurotoxin

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 1X PBS (pH 7.2) containing 20% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Clostridium Botulinum Toxin A Antibody - Azide and BSA Free

• Atx
• Bna
• BoNT/A
• Bontoxilysin A
• BotA
• BOTOX
• Botulinum neurotoxin type A
• Botulism A toxoid

Background

Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Clostridium Botulinum Toxin A Antibody (NB110-17098). (Showing 1 - 2 of 2 FAQ).

1. We purchased Clostridium Botulinum Toxin A Antibody. Is there a specific western blot protocol for this antibody?
   • Unfortunately we do not have a product specific protocol for this one. If we had additional information for special conditions we would have that protocol listed under the product.
2. We would like to know LOD (limit of detection) numerical value of NB110-17098.
   • NB110-17098 - We have not done any assay optimization, so we are not able to tell what kind of limit of detection it is possible to achieve. It will depend a lot on the specific application used and the optimization done with it.