Clenbuterol Antibody (PSH0-01) - Azide and BSA Free

ELISA: Clenbuterol Antibody (PSH0-01) [NBP3-32191] - Indirect ELISA analysis of Clenbuterol was performed by coating wells of a 96-well plate with 50 µl per well of Clenbuterol-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of Clenbuterol monoclonal antibody serial diluted starting from a concentration of 20ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

• Reactivity: All-NA
• Applications: ELISA
• Clone: PSH0-01
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Clenbuterol Antibody (PSH0-01) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Clenbuterol-OVA
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4)
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Clenbuterol Antibody (PSH0-01) - Azide and BSA Free

• 1-(4-Amino-3,5-dichlorophenyl)-2-(tert-butylamino)ethanol
• 37148-27-9
• 4-Amino-3,5-dichloro-alpha-(((1,1-dimethylethyl)amino)methyl)benzenemethanol
• Clenbuterolum
• NAB 365
• Planipart

Background

Clenbuterol belongs to the group of agonists. In livestock production clenbuterol improves the meat/fat ratio in fattened animals or accelerate the growth. Up to now agonists have not been authorized as adjuvants for fattening. In addition to its lipolytic and anabolic effect, clenbuterol has a relaxing effect on non-striated musculature on which is based its therapeutic use as an antiasthmatic and a tocolytic agent. When employed as a fattening adjuvant, as compared with the therapeutic use, clenbuterol is administered in a 5 to 10 times higher dose. Therefore, it is possible that clenbuterol residues may lead to a risk for consumers after illegal administration.Using the clenbuterol monocalantibody, it is possible to detect clenbuterol and other agonists in urine, muscle and liver both rapidly and with accuracy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.