CLEC2L Antibody (2326G) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with human CLEC2L and eGFP was stained with (A) Rabbit Anti-Human CLEC2L Monoclonal Antibody (Catalog # MAB2531) or (B) Rabbit IgG control antibody (MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 2326G
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CLEC2L Antibody (2326G) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human embryonic kidney cell, HEK293-derived human CLEC2L protein
  Ser90-Thr214
  Accession # P0C7M8-1
• Specificity: Detects human CLEC2L in direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC2L Antibody (2326G) [Unconjugated]

• CLEC2L
• C-type lectin domain family 2 member L
• C-type lectin domain family 2, member L

Background

C-type lectin domain family 2, member L (CLEC2L), is a type II transmembrane protein of the CLEC2 family of proteins that includes CD69, LLT1, AICL and KACL (1, 2). CLEC2 family members have broad tissue expression, with LLT1, AICL and KACL expressed on B cells, monocytes, and keratinocytes, respectively (3-5). In contrast, CLEC2L, a homodimeric cell surface receptor, is predominantly expressed in the brain and is referred to as the brain-associated C-type lectin (BACL) (6). Mature human CLEC2L includes a 68 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 125 aa extracellular domain (ECD). Within the ECD, human CLEC2L shares 94% aa sequence identity with mouse and rat CLEC2L. Our assay shows that CLEC2L binds to Galectin-3.

1. Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
2. Vogler, I. and A. Steinle (2011) J. Innate Immune. 3:227.
3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
4. Spreu, J. et al. (2010) Proc. Natl. Acad. Sci. USA 107:5100.
5. Rosen, D.B. et al. (2008) J. Immunol. 180:6508.
6. Lysenko, O. et al. (2013) PloS One. 8:e65345.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P0C7M8-1()