ChABC Antibody (6A12) - BSA Free

Western Blot: ChABC Antibody (6A12) [NBP1-96142] - Western blot analysis of ChABC using NBP1-96141 (ChABC/1E10) and NBP1-96142 (ChABC/6A12). See Notes for additional information.

• Reactivity: All-NA
• Applications: WB, ELISA
• Clone: 6A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ChABC Antibody (6A12) - BSA Free Summary

• Description: Novus Biologicals Mouse ChABC Antibody (6A12) - BSA Free (NBP1-96142) is a monoclonal antibody validated for use in WB and ELISA. Anti-ChABC Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Chondroitinase ABC
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: This ChABC (6A12) antibody is useful for ELISA and Western blot, where a band can be seen at approximately 80 kDa. A fainter band can be seen around 90 kDa. It can be used in Sandwich ELISA as the capture antibody with catalog # NBP1-96141, ChABC (1E10) as the detection antibody.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Western blot data: 125 ng Chondroitinase ABC was mixed with 100 ug of fetal bovine serum (as a background control). The proteins were mixed with 100 ul of non-reducing sample buffer and applied to a single 6 cm well set in a SDS-PAGE minigel (approx 20 ng/well [0.5 cm]). The gel bands were electroblotted to a nitrocellulose sheet. Immunolabeling for individual antibodies was performed on the sheet placed in a slot blot devise. Affinity purified NBP1-96141 (ChABC/1E10) and NBP1-96142 (ChABC/6A12) were applied at 5 ug/ml IgG.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ChABC Antibody (6A12) - BSA Free

• Chondroitinase ABC

Background

ChABC (Chondroitinase ABC) is a bacterial enzyme that has been used for decades, mostly for biochemical characterization of chondroitin sulfate proteoglycans (CSPG). This enzyme degrades the sugar chains attached to the proteoglycan core protein. More recently, ChABC has been used to therapeutically degrade and inactivate CSPG in vivo. CSPGs are involved in a variety of biological processes and play a major inhibitory role in regeneration in the nervous system. For instance, CSPGs have been implicated in preventing regeneration after spinal cord injury. Injection of ChABC at the site of spinal cord injury has improved recovery in numerous animal models. ChABC antibodies serve as tools to help determine the ChABC distribution and content in tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ChABC Antibody (NBP1-96142)(1)

Publication using NBP1-96142

• Khalil AS, Hellenbrand D, Reichl K et al. A Localized Materials-based Strategy to Non-virally Deliver Chondroitinase ABC mRNA Improves Hindlimb Function in A Rat Spinal Cord Injury Model Advanced healthcare materials 2022-07-26 [PMID: 35882512] (WB, P. vulgaris)

FAQs for ChABC Antibody (NBP1-96142). (Showing 1 - 1 of 1 FAQ).

1. Can you tell me antibodies NBP1-96142 and NBP1-96141 have been tested for use in IHC? I would like use them for cardiac samples from my mouse model.
   • I have taken a look into our data, and unfortunately, neither of the mentioned antibodies have been tested in IHC yet. We have only validated these in WB and ELISA, and while they may very well work in IHC, we cannot guarantee it.