CEP27 Antibody

Images

 
Western Blot: CEP27 Antibody [NBP2-15861] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CEP27 Antibody [NBP2-15861] - A431 cells were fixed in iced-cold MeOH for 5 min. Green: HAUS2 protein stained by HAUS2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CEP27 Antibody Summary

Immunogen
Full length human HAUS2 Recombinant protein.
Localization
Centrosome; Spindle
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HAUS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Monkey (85%), Bovine (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CEP27 Antibody

  • centrosomal protein 27kDa
  • Centrosomal protein of 27 kDa
  • CEP27C15orf25
  • chromosome 15 open reading frame 25
  • FLJ10460
  • HAUS augmin-like complex subunit 2
  • HAUS augmin-like complex, subunit 2
  • HsT17025

Background

HAUS2 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CEP27 Antibody (NBP2-15861) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CEP27 Products

Bioinformatics Tool for CEP27 Antibody (NBP2-15861)

Discover related pathways, diseases and genes to CEP27 Antibody (NBP2-15861). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol HAUS2
Uniprot