CCL23/MPIF-1 Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL23/MPIF-1 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CCL23/MPIF-1 (R&D Systems, Catalog # 371-MP)
  Arg22-Asn120
  Accession # P55773
• Specificity: Detects human CCL23/MPIF-1 in ELISAs and Western blots. In sandwich immunoassays, approximately 10% cross-reactivity with recombinant human (rh) CK beta 8-1 (a splice variant of rhMPIF-1) is observed, and less than 0.1% cross-reactivity with rhHCC-1, rhMIP-1δ, and rhMPIF-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL23
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Human CCL23/MPIF-1 Antibody (Catalog # MAB3711)
  ELISA Detection: Human CCL23/MPIF-1 Biotinylated Antibody (Catalog # BAF371)
  Standard: Recombinant Human CCL23/MPIF-1 (Catalog # 371-MP)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Myeloid progenitor inhibitory factor (MPIF-1), also known as CK beta 8 and MIP-3, is a member of the CC chemokine subfamily that is designated CCL23. Alternative splicing of the MPIF-1 gene results in two mRNAs that encode a short (CK beta 8) and a long (CK beta 8-1) isoform of the chemokine. CK beta 8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CK beta 8 (aa 22 - 120). Additional N-terminal processing of the 99 aa residue variant can generate a 75 aa residue CK beta 8 (aa 46 - 120) that is significantly more active than the 99 aa residue variant. Similarly, CK beta 8-1 encodes a 137 aa residue precursor protein that can give rise to a 116 and a 92 aa residue chemokine. Among CC chemokine members, MPIF-1 is most closely related to MIP-5/CCL15 (67% sequence identity) and MIP-1 alpha /CCL3 (51%). MPIF-1 mRNA is most abundant in the adult lung and liver, but is also present in bone marrow, placenta, and various myelomonocytic cell lines. MPIF-1 has been shown to suppress the low proliferative potential colony-forming cells that give rise to granulocyte and monocyte lineages. MPIF-1 binds to CCR1 with high affinity and has been shown to be a potent chemoattractant and activator of monocytes, dendritic cells, and osteoclast precursors.

1. Patel, V. et al. (1997) J. Exp. Med. 185:1163.
2. Youn, B-S. et al. (1998) Blood 91:3118.
3. Nardelli, B. et al. (1999) J. Leukoc. Biol. 61:822.
4. Berkhout, T.A. et al. (2000) Biochem. Pharmacol. 59:591.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL23