Caspase-11/Caspase-4 Antibody (919916) [Unconjugated]

Caspase-11/Caspase-4 was detected in immersion fixed mouse splenocytes treated with LPS using Rat Anti-Mouse Caspase-11/Caspase-4 Monoclonal Antibody (Catalog # MAB86481) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: ICC/IF
• Clone: 919916
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Caspase-11/Caspase-4 Antibody (919916) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Caspase-11/Caspase-4
  Pro81-Glu266
  Accession # P70343
• Specificity: Detects mouse Caspase-11/Caspase-4 in direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Casp4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-11/Caspase-4 Antibody (919916) [Unconjugated]

• CASP11
• Casp11;CASP-11;Casp4;CASP-4;Caspl;Ich3;ich-3
• Caspase-11
• Caspl
• Ich-3

Background

Mouse Caspase-11 (commonly referred as Caspase-4) is a member of the cysteine-aspartic acid protease family known as caspases that function in initiating apoptosis or activating proinflammitory cytokines. Mouse Caspase-11 is expressed as a 343 amino acid latent zymogen. The active caspase-11 is cleaved into two subunits, p20 (aa 81-266) and p10 (aa 286-373).  Caspase-11 is one of several caspases associated with the inflammasome and plays an important role in the activation of proinflammatory cytokines. Caspase-11 is activated by an autocatalytic mechanism or by cleavage by Caspase-8 in response to ER stress or bacterial infection, and its expression and activation can be induced by exposure to LPS. The active enzyme can cleave and activate Caspase-1 which in turn activates IL-1 beta. Mouse Caspase-11 has a 59 and 89% homology to human and rat Caspase-4, respectively. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-11/Caspase-4 Antibody (MAB86481)(1)

Publication using MAB86481

• Shi, Y;He, T;Liu, H;Li, X;Li, Z;Wen, Q;Dai, Z;Sun, X;Tan, Q;Yang, W;Jiang, Y;Liu, Y;Yuan, H;Lei, F;Yi, Y;Cai, J; Ganglioside GA2-mediated caspase-11 activation drives macrophage pyroptosis aggravating intimal hyperplasia after arterial injury International journal of biological sciences 2025-01-01 [PMID: 39744431] (Immunohistochemistry, Transgenic Mouse)

Bioinformatics

• Gene Symbol: Casp4
• Uniprot:
  • P70343()