BoNT-B Light Chain Antibody (579631)

Images

 
There are currently no images for BoNT-B Light Chain Antibody (MAB5420).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity CbSpecies Glossary
Applications WB
Clone
579631
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

BoNT-B Light Chain Antibody (579631) Summary

Immunogen
E. coli-derived recombinant Clostridium BoNT-B Light Chain
Pro2-His428
Accession # P10844
Specificity
Detects C. botulinum BoNT-B Light Chain in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant Light Chains of BoNT-A, -C1, -D, -E, -F, or -G is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
boNT/B
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Reactivity Notes

C. botulinum

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BoNT-B Light Chain Antibody (579631)

  • BoNTB Light Chain
  • BoNT-B Light Chain

Background

Botulinum Neurotoxin Type B is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2-4).

  1. Campbell K.D. et al. (1993) J. Clin. Microbiol. 31:2255.
  2. Montecucco, C. and Giampietro, S. (1993) Trends Biochem. Sci. 18:324.
  3. Turton, K. et al. (2002) Trends Biochem. Sci. 27:552.
  4. Schiavo, G. et al. (2000) Physiol. Rev. 80:717.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BoNT-B Light Chain Antibody (MAB5420) (0)

There are no publications for BoNT-B Light Chain Antibody (MAB5420).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for BoNT-B Light Chain Antibody (MAB5420) (0)

There are no reviews for BoNT-B Light Chain Antibody (MAB5420). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BoNT-B Light Chain Antibody (MAB5420) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional BoNT-B Light Chain Products

Bioinformatics Tool for BoNT-B Light Chain Antibody (MAB5420)

Discover related pathways, diseases and genes to BoNT-B Light Chain Antibody (MAB5420). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on BoNT-B Light Chain

There are no specific blogs for BoNT-B Light Chain, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BoNT-B Light Chain Antibody (579631) and receive a gift card or discount.

Bioinformatics

Gene Symbol boNT/B
Uniprot
Novus 100% Guarantee