![ELISA: Birch pollen Bet V1 Antibody (M0418) - Chimeric [NBP3-09013] - ELISA Plate coated with Birch pollen Bet V1 at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP3-09013. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used. Absorbance at 450nm does not reach a plateau but falls slightly before increasing once more. This is suggestive of two binding sites on Birch pollen Bet V1 for which NBP3-09013 has differing affinities.](http://images.novusbio.com/fullsize/Birch-pollen-Bet-V1-Antibody-M0418-Chimeric-ELISA-NBP3-09013-img0001.jpg "ELISA: Birch pollen Bet V1 Antibody (M0418) - Chimeric [NBP3-09013] - ELISA Plate coated with Birch pollen Bet V1 at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP3-09013. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used. Absorbance at 450nm does not reach a plateau but falls slightly before increasing once more. This is suggestive of two binding sites on Birch pollen Bet V1 for which NBP3-09013 has differing affinities.")
| Reactivity | PlSpecies Glossary |
| Applications | ELISA, WB |
| Clone | M0418 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit Birch pollen Bet V1 Antibody (M0418) - Chimeric - Azide and BSA Free (NBP3-09013) is a recombinant monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Bet v 1-A, a major allergen of the European white birch. |
| Specificity | This antibody binds to Bet v 1, in particular to the naturally occurring isoforms Bet v 1.0101 and Bet v 1.0102, but not to Bet v 1.0112. |
| Isotype | IgG Lambda |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to Bet v 1 via a charaterized epitope and has been used in crystallographic studies. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
