![ELISA: Birch pollen Bet V1 Antibody (BV16) [NBP2-81093] - Binding curve of [NBP2-81093] to Bet v 1. ELISA Plate coated with Bet v 1-A at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-81093. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used. Absorbance at 450nm does not reach a plateau but falls slightly before increasing once more. This is suggestive of two binding sites on Bet v 1-A for which NBP2-81093 has differing affinities.](http://images.novusbio.com/fullsize/Birch-pollen-Bet-V1-Antibody-BV16-ELISA-NBP2-81093-img0001.jpg "ELISA: Birch pollen Bet V1 Antibody (BV16) [NBP2-81093] - Binding curve of [NBP2-81093] to Bet v 1. ELISA Plate coated with Bet v 1-A at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-81093. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used. Absorbance at 450nm does not reach a plateau but falls slightly before increasing once more. This is suggestive of two binding sites on Bet v 1-A for which NBP2-81093 has differing affinities.")
| Reactivity | PlSpecies Glossary |
| Applications | WB, ELISA |
| Clone | BV16 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit Birch pollen Bet V1 Antibody (BV16) - Chimeric - Azide and BSA Free (NBP2-81093) is a recombinant monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Birch pollen Bet V1-A, a major allergen of the European white birch. |
| Specificity | This antibody binds to Birch pollen Bet V1. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to Bet v 1 via an epitope commonly used by allergogenic IgE. Fab versions of this antibody have been used in co-crystallization studies of Bet v 1. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
