Bacillus anthracis protective antigen Antibody

Western Blot: Bacillus anthracis protective antigen Antibody [NB120-13808] - Analysis of Bacillus anthracis protective antigen in recombinant protein using Bacillus anthracis protective antigen antibody (Lane B) at 1:1000 dilution. Lane A shows the pre-bleed.

• Reactivity: Ba
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Bacillus anthracis protective antigen Antibody Summary

• Description: Novus Biologicals Rabbit Bacillus anthracis protective antigen Antibody (NB120-13808) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 240-254 of the Anthrax PA protein (~83 kDa).
• Specificity: PA is cleaved from the 83 kDa form to the 63 kDa form at amino acids 164-167. Thus the (made against amino acids 250-250) should recognize both the 83 kDa and 63 kDa PA forms, but not the 20 kDa cleavage fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000

Reactivity Notes

Anthrax PA produced by Bacillus anthracis

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Bacillus anthracis protective antigen Antibody

• anthrax PA
• Anthrax toxins translocating protein
• PA 83
• PA
• PA83
• PAG
• Paga
• Protective antigen

Background

Anthrax toxins are composed of three distinct proteins, a protective antigen (PA), a lethal factor (LF) and an edema factor (EF). None of these proteins are toxic by themselves and several studies indicate that the anthrax toxin has the familiar A-B enzymatic binding structure, with PA acting as the binding domain and EF and/or LF acting as the active fragments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.