![Western Blot: Aurora A/B/C Antibody (RM454)[NBP3-18823]Western Blot of the lysates of HeLa cells nontreated (-) or treated (+) with Nocodazole and Calyculin A, using Anti-Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) rabbit monoclonal antibody (clone RM454) at a 1:2500 dilution.](http://images.novusbio.com/fullsize/nbp3-18823_rabbit-aurora-a-b-c-mab-rm454-16112023130180.png "Western Blot: Aurora A/B/C Antibody (RM454)[NBP3-18823]Western Blot of the lysates of HeLa cells nontreated (-) or treated (+) with Nocodazole and Calyculin A, using Anti-Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) rabbit monoclonal antibody (clone RM454) at a 1:2500 dilution.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clone | RM454 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit Aurora A/B/C Antibody (RM454) (NBP3-18823) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A phospho-peptide corresponding to Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (Uniprot # O14965, Q96GD4, Q9UQB9) |
| Specificity | This antibody reacts to human Aurora A/B/C only when phosphorylated at either Thr288, Thr232 or Thr198 respectively. There is no cross-reactivity to Aurora A/B/C that is not phosphorylated. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: Aurora A/B/C Antibody (RM454) [NBP3-18823] - Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section, using clone RM454 at a 1:50 dilution.](http://images.novusbio.com/fullsize/nbp3-18823_rabbit-aurora-a-b-c-mab-rm454-immunohistochemistry-paraffin-18920252343564.png "Immunohistochemistry-Paraffin: Aurora A/B/C Antibody (RM454) [NBP3-18823] - Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section, using clone RM454 at a 1:50 dilution.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")