![Western Blot: Aurora A/B/C Antibody (4A7) [NBP2-50046] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50046. Nocodazole is a microtubule depolymerizating agent which induces cells to halt at the G2/M phase and also induces Aurora kinase expression. NBP2-50046 binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B. Also, it recognizes a weak band at 35 kDa which is Aurora C. Right: Blot of recombinant full length human Aurora A, B and C was probed with NBP2-50046.](http://images.novusbio.com/fullsize/Aurora-A-B-C-Antibody-4A7-Western-Blot-NBP2-50046-img0004.jpg "Western Blot: Aurora A/B/C Antibody (4A7) [NBP2-50046] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50046. Nocodazole is a microtubule depolymerizating agent which induces cells to halt at the G2/M phase and also induces Aurora kinase expression. NBP2-50046 binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B. Also, it recognizes a weak band at 35 kDa which is Aurora C. Right: Blot of recombinant full length human Aurora A, B and C was probed with NBP2-50046.")
| Reactivity | Hu, Mu, Rt, Po, Bv, EqSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 4A7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Full length recombinant human AURKA protein expressed from E. coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In WB, this antibody binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B respectively. It also recognizes a weak band at 35 kDa which corresponds to Aurora C. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Aurora A/B/C Antibody (4A7) [NBP2-50046] - HeLa cell cultures were stained with NBP2-50046 (green). In this image, strong staining in spindle poles was seen in cells at anaphase. This antibody also stains the midbodies between the two daughter cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-A-B-C-Antibody-4A7-Immunocytochemistry-Immunofluorescence-NBP2-50046-img0003.jpg "Immunocytochemistry/Immunofluorescence: Aurora A/B/C Antibody (4A7) [NBP2-50046] - HeLa cell cultures were stained with NBP2-50046 (green). In this image, strong staining in spindle poles was seen in cells at anaphase. This antibody also stains the midbodies between the two daughter cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.")
![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")