![Western Blot: Aurora A/B Antibody (5A12) [NBP2-50049] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A/B mAb. Left: cells were treated with 100 ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: western blot of purified full length human recombinant aurora A, B, C preparations as indicated. Bands at 46kDa and 38kDa correspond to aurora A and B proteins but not C. NBP2-50049 reacts with aurora A an B but not C.](http://images.novusbio.com/fullsize/Aurora-A-B-Antibody-5A12-Western-Blot-NBP2-50049-img0004.jpg "Western Blot: Aurora A/B Antibody (5A12) [NBP2-50049] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A/B mAb. Left: cells were treated with 100 ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: western blot of purified full length human recombinant aurora A, B, C preparations as indicated. Bands at 46kDa and 38kDa correspond to aurora A and B proteins but not C. NBP2-50049 reacts with aurora A an B but not C.")
| Reactivity | Hu, Mu, Rt, Ca, EqSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 5A12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Mouse Aurora A/B Antibody (5A12) - BSA Free (NBP2-50049) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Full length recombinant human Aurora A protein expressed in and purified from E. coli. [UniProt# O14965] |
| Specificity | This antibody does not cross-react with Aurora C. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In WB, this antibody binds strongly to Aurora A at about 46 kDa and weakly to Aurora B at 38 kDa. |
| Theoretical MW | 46, 38 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Aurora A/B Antibody (5A12) [NBP2-50049] - HeLa cell cultures were stained with NBP2-50049 (green). The antibody stains spindle poles and mitotic spindles at anaphase (top 2 panels) and concentrates on the midbody between the two daughter cells during telophase (bottom 2 panels). It is therefore a useful marker of dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-A-B-Antibody-5A12-Immunocytochemistry-Immunofluorescence-NBP2-50049-img0001.jpg "Immunocytochemistry/Immunofluorescence: Aurora A/B Antibody (5A12) [NBP2-50049] - HeLa cell cultures were stained with NBP2-50049 (green). The antibody stains spindle poles and mitotic spindles at anaphase (top 2 panels) and concentrates on the midbody between the two daughter cells during telophase (bottom 2 panels). It is therefore a useful marker of dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")