![Western Blot: Aurora A/B Antibody (3H1) [NBP2-50043] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A/B mAb. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase, for 6hrs prior western blotting: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] murine KR158 cells. Right: western blot of purified full length human recombinant aurora A, B, C as indicated. Bands at 46kDa and 38kDa correspond to aurora A and B proteins respectively, showing that NBP2-50043 reacts with these to proteins but not aurora C.](http://images.novusbio.com/fullsize/Aurora-A-B-Antibody-3H1-Western-Blot-NBP2-50043-img0005.jpg "Western Blot: Aurora A/B Antibody (3H1) [NBP2-50043] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A/B mAb. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase, for 6hrs prior western blotting: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] murine KR158 cells. Right: western blot of purified full length human recombinant aurora A, B, C as indicated. Bands at 46kDa and 38kDa correspond to aurora A and B proteins respectively, showing that NBP2-50043 reacts with these to proteins but not aurora C.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 3H1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Mouse Aurora A/B Antibody (3H1) - BSA Free (NBP2-50043) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant full length human Aurora A expressed in and purified from E. coli. [UniProt# O14965] |
| Specificity | This antibody does not cross-react with Aurora C. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In WB, this antibody reacts with a band at 46 kDa corresponding to Aurora A and weakly reacts with a band at 38 kD corresponding to Aurora B. In ICC/IF, Aurora A localizes in spindle poles and mitotic spindles at anaphase, while Aurora B localizes in midzone at metaphase and concentrates on the midbody between the two daughter cells during telephase. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Aurora A/B Antibody (3H1) [NBP2-50043] - HeLa cell cultures were stained with NBP2-50043 (green). The antibody stains spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-A-B-Antibody-3H1-Immunocytochemistry-Immunofluorescence-NBP2-50043-img0003.jpg "Immunocytochemistry/Immunofluorescence: Aurora A/B Antibody (3H1) [NBP2-50043] - HeLa cell cultures were stained with NBP2-50043 (green). The antibody stains spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")