Allophycocyanin Antibody (APC-6A2)

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Flow Cytometry: Allophycocyanin Antibody (APC-6A2) [NBP2-00151] - Staining of normal human peripheral blood cells with Rat Anti-Human/Mouse CD44 APC followed by 0.015 ug of Mouse IgG1 kappa Isotype Control Purified ...read more

Product Details

Summary
Reactivity NASpecies Glossary
Applications Flow
Clone
APC-6A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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Catalog# & Conjugate Size Price

Allophycocyanin Antibody (APC-6A2) Summary

Immunogen
The immunogen for this antibody is Allophycocyanin.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This APC-6A2 antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.015 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Allophycocyanin Antibody (APC-6A2)

  • Allophycocyanin
  • APC

Background

This antibody reacts with allophycocyanin (APC) and APC-Cy7, with no crossreactivity to fluorescein (FITC) or phycoerythrin (PE). When using APC-6A2 as a second-step antibody, in combination with an appropriate third step, to bind a APC conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated APC antibody. Therefore no interference or quenching of the APC fluorochrome is observed. Th oAPC-6A2 can be used for separation of cells labeled with APCconjugated antibodies or for staining.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP2-00147

Additional Allophycocyanin Products

Allophycocyanin NBP2-00151

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