![Flow Cytometry: Allophycocyanin Antibody (APC-6A2) [NBP2-00151] - Staining of normal human peripheral blood cells with Rat Anti-Human/Mouse CD44 APC followed by 0.015 ug of Mouse IgG1 kappa Isotype Control Purified (left) or 0.015 ug of Anti-Allophycocyanin Purified (right) followed by detection with Anti-Mouse IgG FITC . Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Allophycocyanin-Antibody-APC-6A2-Flow-Cytometry-NBP2-00151-img0001.jpg "Flow Cytometry: Allophycocyanin Antibody (APC-6A2) [NBP2-00151] - Staining of normal human peripheral blood cells with Rat Anti-Human/Mouse CD44 APC followed by 0.015 ug of Mouse IgG1 kappa Isotype Control Purified (left) or 0.015 ug of Anti-Allophycocyanin Purified (right) followed by detection with Anti-Mouse IgG FITC . Total viable cells were used for analysis.")
| Reactivity | Hu, All-NASpecies Glossary |
| Applications | Flow |
| Clone | APC-6A2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.2 mg/ml |
| Description | Novus Biologicals Mouse Allophycocyanin Antibody (APC-6A2) - BSA Free (NBP2-00151) is a monoclonal antibody validated for use in Flow. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen for this antibody is Allophycocyanin. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This APC-6A2 antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.015 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.2) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")