![ELISA: African Swine Fever Virus p54 Antibody (HL1219) - Azide and BSA Free [NBP3-25367] - Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below. Capture: ASFV p54 antibody [HL1218] (NBP3-25366) (5 ug/mL) Detection: HRP-conjugated ASFV p54 antibody [HL1219] (NBP3-25367) (0.1 ug/mL) Please notice that NBP3-25367 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25366. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25367_rabbit-african-swine-fever-virus-p54-mab-hl1219-azide-bsa-free-2120249355548.jpg "ELISA: African Swine Fever Virus p54 Antibody (HL1219) - Azide and BSA Free [NBP3-25367] - Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below. Capture: ASFV p54 antibody [HL1218] (NBP3-25366) (5 ug/mL) Detection: HRP-conjugated ASFV p54 antibody [HL1219] (NBP3-25367) (0.1 ug/mL) Please notice that NBP3-25367 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25366. Please contact us for custom HRP-conjugated antibody.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, LFA |
| Clone | HL1219 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of African Swine Fever Virus p54. The exact sequence is proprietary. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | E183L |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | E183L |
![Immunocytochemistry/Immunofluorescence: African Swine Fever Virus p54 Antibody (HL1219) - Azide and BSA Free [NBP3-25367] - ASFV p54 antibody [HL1219] detects ASFV p54 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASFV p54 stained by ASFV p54 antibody [HL1219] (NBP3-25367) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25367_rabbit-african-swine-fever-virus-p54-mab-hl1219-azide-bsa-free-212024939174.jpg "Immunocytochemistry/Immunofluorescence: African Swine Fever Virus p54 Antibody (HL1219) - Azide and BSA Free [NBP3-25367] - ASFV p54 antibody [HL1219] detects ASFV p54 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASFV p54 stained by ASFV p54 antibody [HL1219] (NBP3-25367) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.")
![Western Blot: African Swine Fever Virus p54 Antibody (HL1219) - Azide and BSA Free [NBP3-25367] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1219] (NBP3-25367) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25367_rabbit-african-swine-fever-virus-p54-mab-hl1219-azide-bsa-free-212024940848.jpg "Western Blot: African Swine Fever Virus p54 Antibody (HL1219) - Azide and BSA Free [NBP3-25367] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1219] (NBP3-25367) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: African Swine Fever Virus p54 Antibody (HL1219) - Azide and BSA Free [NBP3-25367] - Indirect ELISA analysis performed by coating plate with recombinant recombinant HEK293 expressed, full-length ASFV p54 (40-0.63 nM). Coated protein was probed with ASFV p54 antibody [HL1219] (NBP3-25367) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25367_rabbit-african-swine-fever-virus-p54-mab-hl1219-azide-bsa-free-212024941420.jpg "ELISA: African Swine Fever Virus p54 Antibody (HL1219) - Azide and BSA Free [NBP3-25367] - Indirect ELISA analysis performed by coating plate with recombinant recombinant HEK293 expressed, full-length ASFV p54 (40-0.63 nM). Coated protein was probed with ASFV p54 antibody [HL1219] (NBP3-25367) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect bound primary antibody.")
![Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1219) - Azide and BSA Free [NBP3-25367] - Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (NBP3-25367 [HL1219]) Detection: ASFV p54 antibody (NBP3-25370 [HL1289]) Samples (20 ng) : 1. ASFV p54 (ECD) protein 2. Lysis buffer](http://images.novusbio.com/fullsize/nbp3-25367_rabbit-african-swine-fever-virus-p54-mab-hl1219-azide-bsa-free-2120249415914.jpg "Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1219) - Azide and BSA Free [NBP3-25367] - Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (NBP3-25367 [HL1219]) Detection: ASFV p54 antibody (NBP3-25370 [HL1289]) Samples (20 ng) : 1. ASFV p54 (ECD) protein 2. Lysis buffer")
![Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1219) - Azide and BSA Free [NBP3-25367] - Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (NBP3-25367 [HL1219]) Detection: ASFV p54 antibody (NBP3-25366 [HL1218]) Samples (20 ng) : 1. ASFV p54 (ECD) protein 2. Lysis buffer](http://images.novusbio.com/fullsize/nbp3-25367_rabbit-african-swine-fever-virus-p54-mab-hl1219-azide-bsa-free-2120249432845.jpg "Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1219) - Azide and BSA Free [NBP3-25367] - Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (NBP3-25367 [HL1219]) Detection: ASFV p54 antibody (NBP3-25366 [HL1218]) Samples (20 ng) : 1. ASFV p54 (ECD) protein 2. Lysis buffer")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")