A2B5 Antibody (105) [Biotin]

NTera-2 human testicular embryonic carcinoma cell line either treated with 10 µM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Biotinylated Monoclonal Antibody (Catalog # BAM1416), followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: Flow
• Clone: 105
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

A2B5 Antibody (105) [Biotin] Summary

• Immunogen: Embryonic chicken retinal cells
• Specificity: Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: IgM-specific Affinity-purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for A2B5 Antibody (105) [Biotin]

• A2B5 marker
• A2B5

Background

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).

1. Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
2. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
3. Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
4. Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for A2B5 Antibody (BAM1416)(1)

Publication using BAM1416

• Skardelly M, Hempel E, Hirrlinger J, Wegner F, Meixensberger J, Milosevic J Fluorescent protein-expressing neural progenitor cells as a tool for transplantation studies. PLoS ONE, 2014-06-16;9(6):e99819. 2014-06-16 [PMID: 24932758] (Flow Cytometry, Mouse)