A2B5 Antibody (105) [Unconjugated]

Images

 
A2B5 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line (differentiated with retinoic acid for 10 days) using Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Monoclonal Antibody ...read more
NTera‑2 human testicular embryonic carcinoma cell line either treated with 10 μM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
105
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

A2B5 Antibody (105) [Unconjugated] Summary

Immunogen
Embryonic chicken retinal cells
Specificity
Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).
Details of Functionality
ActivityAssaywCitation not found None None
Source
N/A
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB1416 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for A2B5 Antibody (105) [Unconjugated]

  • A2B5

Background

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).
  1. Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
  2. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
  3. Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
  4. Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for A2B5 Antibody (MAB1416)(18)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC, IHC-Fr.


Filter By Application
Flow Cytometry
(4)
ICC
(10)
IHC
(2)
IHC-Fr
(2)
All Applications
Filter By Species
Human
(8)
Mouse
(5)
Rat
(4)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using MAB1416 Applications Species
JP Zepecki, KM Snyder, MM Moreno, E Fajardo, A Fiser, J Ness, A Sarkar, SA Toms, N Tapinos Regulation of human glioma cell migration, tumor growth, and stemness gene expression using a Lck targeted inhibitor Oncogene, 2018;0(0):. 2018 [PMID: 30353164] (IHC, Rat) IHC Rat
JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency Cell Rep, 2017;19(1):20-35. 2017 [PMID: 28380358] (Flow Cytometry, Human) Flow Cytometry Human
MY Cha, YW Kwon, HS Ahn, H Jeong, YY Lee, M Moon, SH Baik, DK Kim, H Song, EC Yi, D Hwang, HS Kim, I Mook-Jung Protein-Induced Pluripotent Stem Cells Ameliorate Cognitive Dysfunction and Reduce A? Deposition in a Mouse Model of Alzheimer's Disease Stem Cells Transl Med, 2017;6(1):293-305. 2017 [PMID: 28170178] (ICC, Mouse) ICC Mouse
Moyon S, Dubessy A, Aigrot M, Trotter M, Huang J, Dauphinot L, Potier M, Kerninon C, Melik Parsadaniantz S, Franklin R, Lubetzki C Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration. J Neurosci, 2015;35(1):4-20. 2015 [PMID: 25568099] (IHC-Fr, Mouse) IHC-Fr Mouse
Leite C, Silva N, Mendes S, Ribeiro A, de Faria J, Lourenco T, dos Santos F, Andrade P, Cardoso C, Vieira M, Paiva A, da Silva C, Cabral J, Relvas J, Graos M Differentiation of human umbilical cord matrix mesenchymal stem cells into neural-like progenitor cells and maturation into an oligodendroglial-like lineage. PLoS ONE, 2014;9(10):e111059. 2014 [PMID: 25357129] (IHC-Fr, Mouse) IHC-Fr Mouse
Robinson A, Rodgers J, Goings G, Miller S Characterization of oligodendroglial populations in mouse demyelinating disease using flow cytometry: clues for MS pathogenesis. PLoS ONE, 2015;9(9):e107649. 2015 [PMID: 25247590] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Macas J, Ku M, Nern C, Xu Y, Buhler H, Remke M, Synowitz M, Franz K, Seifert V, Plate K, Kettenmann H, Glass R, Momma S Generation of neuronal progenitor cells in response to tumors in the human brain. Stem Cells, 2014;32(1):244-57. 2014 [PMID: 24170295] (IHC, Human) IHC Human
Najm, Fadi J, Lager, Angela M, Zaremba, Anita, Wyatt, Krysta, Caprariello, Andrew V, Factor, Daniel C, Karl, Robert T, Maeda, Tadao, Miller, Robert H, Tesar, Paul J Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol, 2013;31(5):426-33. 2013 [PMID: 23584611] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Werbowetski-Ogilvie TE, Morrison LC, Fiebig-Comyn A, Bhatia M In vivo generation of neural tumors from neoplastic pluripotent stem cells models early human pediatric brain tumor formation. Stem Cells, 2012;30(3):392-404. 2012 [PMID: 22213600] (Flow Cytometry, Human) Flow Cytometry Human
Seshadri S, Kamiya A, Yokota Y, Prikulis I, Kano S, Hayashi-Takagi A, Stanco A, Eom TY, Rao S, Ishizuka K, Wong P, Korth C, Anton ES, Sawa A Disrupted-in-Schizophrenia-1 expression is regulated by beta-site amyloid precursor protein cleaving enzyme-1-neuregulin cascade. Proc. Natl. Acad. Sci. U.S.A., 2010;107(12):5622-7. 2010 [PMID: 20212127] (ICC, Human) ICC Human
Show All 18 Publications.

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