![Dot Blot: 5-Formylcytosines Antibody (RM477)[NBP3-18864]Dot blot of DNA using anti-5-fC Rabbit Monoclonal Antibody, Clone RM477. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of linear denatured DNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).](http://images.novusbio.com/fullsize/nbp3-18864_rabbit-5-formylcytosines-mab-rm477-161120231301822.png "Dot Blot: 5-Formylcytosines Antibody (RM477)[NBP3-18864]Dot blot of DNA using anti-5-fC Rabbit Monoclonal Antibody, Clone RM477. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of linear denatured DNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).")
| Reactivity | VbSpecies Glossary |
| Applications | DB, ELISA |
| Clone | RM477 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit 5-Formylcytosines Antibody (RM477) (NBP3-18864) is a recombinant monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | KLH-conjugated 5-Formylcytosines |
| Specificity | This antibody reacts to 5-Formylcytosines in DNA. No cross reactivity with non-modified cytosine and other modified cytosines in DNA. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![ELISA: 5-Formylcytosines Antibody (RM477) [NBP3-18864] - ELISA of DNA using Clone RM477 (1 ug/mL). The plate was coated with different concentrations of linear denatured DNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).](http://images.novusbio.com/fullsize/nbp3-18864_rabbit-5-formylcytosines-mab-rm477-elisa-189202523374015.png "ELISA: 5-Formylcytosines Antibody (RM477) [NBP3-18864] - ELISA of DNA using Clone RM477 (1 ug/mL). The plate was coated with different concentrations of linear denatured DNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")