5-Fluorocytosine Antibody

Immunoprecipitation: 5-Fluorocytosine Antibody [NBP2-59147] - DIP results obtained with the antibody directed against 5-fC.HEK293 cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector. DIP assays were performed on 4 ug of sheared and denatured DNA using 3 uL of the antibody against 5-fC in a total of 500 uL IP buffer. QPCR was performed with primers specific for the reporter gene. Figure shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).

ELISA: 5-Fluorocytosine Antibody [NBP2-59147] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against 5-fC. The plates were coated with the immunogen. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:100,000.

• Reactivity: Hu, Mu
• Applications: ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

5-Fluorocytosine Antibody Summary

• Immunogen: The exact immunogen is propietary information.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunoprecipitation 3 uL/IP

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Serum
• Preservative: 0.05% Sodium Azide
• Purity: Whole Antiserum

Alternate Names for 5-Fluorocytosine Antibody

• 5-fC

Background

5-fluorocytosine (5-FC), is an antifungal medication. It is specifically used, together with amphotericin B, for serious Candida infections and cryptococcosis. It may be used by itself or with other antifungals for chromomycosis. Flucytosine is used by mouth and by injection into a vein. Common side effects include bone marrow suppression, loss of appetite, diarrhea, vomiting, and psychosis. anaphylaxis and other allergic reactions occasionally occur. It is unclear if use in pregnancy is safe for the baby. Flucytosine is in the fluorinated pyrimidine analogue family of medications. It works by being converted into fluorouracil inside the fungus which then blocks its ability to make protein. Flucytosine was first made in 1957. It is on the World Health Organization's List of Essential Medicines, the most effective and safe medicines needed in a health system. As of 2016, in the United States the medication cost about 2,000 USD per day while in the United Kingdom it is about 22 USD per day. It is not available in much of the developing world.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.