![ELISA: 25-hydroxy Vitamin D3 Antibody (RM3) [NBP2-61593] - Competitive ELISA data using anti-25-OH Vitamin D3 rabbit monoclonal antibody.](http://images.novusbio.com/fullsize/Vitamin-D3-Antibody-3-ELISA-NBP2-61593-img0001.jpg "ELISA: 25-hydroxy Vitamin D3 Antibody (RM3) [NBP2-61593] - Competitive ELISA data using anti-25-OH Vitamin D3 rabbit monoclonal antibody.")
| Reactivity | All-NASpecies Glossary |
| Applications | ELISA |
| Clone | RM3 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.1 mg/ml |
| Description | Novus Biologicals Rabbit 25-hydroxy Vitamin D3 Antibody (RM3) (NBP2-61593) is a recombinant monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | 25-hydroxy Vitamin D3 BSA conjugate |
| Specificity | This antibody reacts to 25-hydroxy Vitamin D3. It does not cross-react with Vitamin D3, Vitamin D2, and 25-OH Vitamin D2. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Can be used as a capture antibody when paired with 25-OH Vitamin D3 Antibody-antigen Complex Antibody (RM428) (NBP3-18865) |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![ELISA: 25-hydroxy Vitamin D3 Antibody (RM3) [NBP2-61593] - Cross activity data of NBP2-61593 at 100 ng/mL.](http://images.novusbio.com/fullsize/nbp2-61593_rabbit-monoclonal-25-hydroxy-vitamin-d3-antibody-rm3-161020231947101.png "ELISA: 25-hydroxy Vitamin D3 Antibody (RM3) [NBP2-61593] - Cross activity data of NBP2-61593 at 100 ng/mL.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")