Find an isotype control for your experiment. Isotype controls provide an estimate of non-specific binding of an antibody that may occur during an assay. Use the filters to easily sort our isotype control products by species, isotype reactivities, and labels.
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-96891 | |||
| FITC | NBP1-96847 | |||
| HRP | NBP1-97077 | |||
| Rhodamine | NBP1-96850 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDXCH04A488-100 | |||
| Alexa Fluor 546 | DDXCH06A546-100 | |||
| Alexa Fluor 546 | DDXCH04A546-100 | |||
| Alexa Fluor 647 | DDXCH04A647-100 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP1-96825 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP1-96825 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Agarose | NBP1-97145 | |||
| Alkaline Phosphatase | NBP1-97183 | |||
| Biotin | NBP1-96930 | |||
| FITC | NBP1-96866 | |||
| HRP | NBP1-97082 | |||
| Rhodamine | NBP1-96867 | |||
| Texas Red | NBP1-96931 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-97183 | |||
![Western Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777] - SDS-PAGE of Mouse IgG3. Lane 1: Non-Reduced Human IgG. Lane 2: 5uL OPAL Pre-stained Marker MB-210-0500. Lane 3: Reduced Mouse IgG3. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 180-245 kDa, Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Mouse-IgG3-Isotype-Control-MG3-Western-Blot-NBP1-96777-img0003.jpg "Western Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777] - SDS-PAGE of Mouse IgG3. Lane 1: Non-Reduced Human IgG. Lane 2: 5uL OPAL Pre-stained Marker MB-210-0500. Lane 3: Reduced Mouse IgG3. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 180-245 kDa, Reduced at 55, 25 kDa.")
![Dot Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777] - A three-fold serial dilution of Fluorescein conjugated Mouse IgG3 subclass starting at 200ug was spotted onto 0.45 um nitrocellulose. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Mouse-IgG3-Isotype-Control-MG3-Dot-Blot-NBP1-96777-img0002.jpg)
![SDS-PAGE of Mouse IgG3 Isotype Control (MG3). Lane 1: Non-Reduced Human IgG. Lane 2: [5µL] Opal Pre-stained Marker . Lane 3: Reduced Mouse IgG3 Isotype Control (MG3). Load: 1µg per lane. Predicted/Observed size: Non-reduced at 180-245 kDa, Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/nbp1-96777_mouse-mouse-igg3-isotype-control-mg3-1872025818710.jpg)
![Dot Blot: Human IgG F(ab)2 Isotype Control [Biotin] [NBP1-96827] - Dot Blot results of Human IgG F(ab)2 isotype control [Biotin].Antigen: Human IgG F(ab)2 isotype control [Biotin].Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng.Blocking Buffer for 30 minutes at RT.Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30 min at RT. Imaging System ChemiDoc, Filter used: Chemi.](http://images.novusbio.com/fullsize/Human-IgG-F-ab-2-Isotype-Control-Biotin-Dot-Blot-NBP1-96827-img0001.jpg "Dot Blot: Human IgG F(ab)2 Isotype Control [Biotin] [NBP1-96827] - Dot Blot results of Human IgG F(ab)2 isotype control [Biotin].Antigen: Human IgG F(ab)2 isotype control [Biotin].Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng.Blocking Buffer for 30 minutes at RT.Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30 min at RT. Imaging System ChemiDoc, Filter used: Chemi.")
![Dot Blot results of Human IgG F(ab)2 Isotype Control [Biotin]. Antigen: Human IgG F(ab)2 Isotype Control [Biotin]. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. blocking Buffer for 30 minutes at RT. Secondary Antibody: Streptavidin-HRP](http://images.novusbio.com/fullsize/nbp1-96827_human-human-igg-f-ab-2-isotype-control-biotin-23520231750164.jpg)
![SDS-Page: Mouse IgG1 Kappa Isotype Control (MG1K) [NBP1-96983] - SDS-PAGE of Mouse IgG1 Kappa Isotype Control. Lane 1: Mouse IgG1 Kappa Isotype Control, Non-reduced. M: Opal Pre-stained Ladder. Lane 2: Mouse IgG1 Kappa Isotype Control, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced](http://images.novusbio.com/fullsize/Mouse-IgG1-Kappa-Isotype-Control-MG1K-SDS-Page-NBP1-96983-img0002.jpg "SDS-Page: Mouse IgG1 Kappa Isotype Control (MG1K) [NBP1-96983] - SDS-PAGE of Mouse IgG1 Kappa Isotype Control. Lane 1: Mouse IgG1 Kappa Isotype Control, Non-reduced. M: Opal Pre-stained Ladder. Lane 2: Mouse IgG1 Kappa Isotype Control, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced")
![SDS-Page: Mouse IgM Lambda Isotype Control (MML) [NBP1-96976] - SDS-PAGE of Mouse IgM Lambda isotype control (38). Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Lambda isotype control (38). Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab') at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Mouse-IgM-Lambda-Isotype-Control-MML-SDS-Page-NBP1-96976-img0002.jpg "SDS-Page: Mouse IgM Lambda Isotype Control (MML) [NBP1-96976] - SDS-PAGE of Mouse IgM Lambda isotype control (38). Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Lambda isotype control (38). Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab') at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.")
![SDS-Page: Mouse IgM Lambda Isotype Control (MML) [NBP1-96976] - SDS-PAGE of Mouse IgM lambda isotype control. Lane 1: 1x Reduced Mouse IgM lambda. Lane 2: Opal Prestained Standard. Lane 3: 1X Non-reduced Mouse IgM lambda. 4-20% Lonza SDS-PAGE; Coomassie Stained; ChemiDoc Imaged.](http://images.novusbio.com/fullsize/Mouse-IgM-Lambda-Isotype-Control-MML-SDS-Page-NBP1-96976-img0004.jpg)
![Western Blot: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit IgM Isotype Control-Western Blot-NBP1-96968-img0003.jpg "Western Blot: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![SDS-Page: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.](http://images.novusbio.com/fullsize/Rabbit-IgM-Isotype-Control-SDS-Page-NBP1-96968-img0001.jpg)
![Western Blot: Rat IgG Isotype Control [NBP1-97040] - SDS-Page: Rat IgG Isotype Control [Agarose Immobilized] [NBP1-97145] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule Agarose Conjugated. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM. Image using the Agarose form of this antibody.](http://images.novusbio.com/fullsize/Rat-IgG-Isotype-Control-Western-Blot-NBP1-97040-img0002.jpg "Western Blot: Rat IgG Isotype Control [NBP1-97040] - SDS-Page: Rat IgG Isotype Control [Agarose Immobilized] [NBP1-97145] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule Agarose Conjugated. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM. Image using the Agarose form of this antibody.")
![SDS-Page: Rat IgG Isotype Control [NBP1-97040] - SDS PAGE of Rat IgG Whole Molecule. Lane 1: Non-Reduced Rat IgG Whole Molecule. Lane 2: 5 uL Opal Prestained Marker. Lane 3: Reduced Rat IgG Whole Molecule. Load: 1 ug per lane.](http://images.novusbio.com/fullsize/Rat IgG Isotype Control-SDS-Page-NBP1-97040-img0003.jpg)