Human IgG4 Isotype Control [Alexa Fluor® 546]

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clonality: Polyclonal
• Host: Human
• Conjugate: Alexa Fluor 546

Human IgG4 Isotype Control [Alexa Fluor® 546] Summary

• Description: This Human IgA immunoglobulin is useful as an isotype control for Human IgA antibody.
• Isotype: IgA
• Clonality: Polyclonal
• Host: Human
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface) 1:10 - 1:1000
  • Flow (Intracellular) 1:10 - 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunofluorescence 1:10 - 1:500
• Application Notes: Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol.
• Preservative: No Preservative
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Alternate Names for Human IgG4 Isotype Control [Alexa Fluor® 546]

• Immunoglobulin G
• Immunoglobulin G4
• ImmunoglobulinG

Background

Immunoglobulin G (IgG), is one of the most abundant protein in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.