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![Western Blot: BRCA1 Antibody Pack [NB100-936] - Detection of BRCA1 in MCF-7 whole cell lysate using NB 100-600. 12 minute ECL exposure.](http://images.novusbio.com/fullsize/BRCA1-Antibody-Pack-Western-Blot-NB100-936-img0006.jpg "Western Blot: BRCA1 Antibody Pack [NB100-936] - Detection of BRCA1 in MCF-7 whole cell lysate using NB 100-600. 12 minute ECL exposure.")
![Western Blot: BRCA1 Antibody Pack [NB100-936] - Detection of BRCA1 in MCF-7 whole cell lysate using NB 100-598, 1 minute ECL detection.](http://images.novusbio.com/fullsize/BRCA1-Antibody-Pack-Western-Blot-NB100-936-img0001.jpg)
![Western Blot: BRCA1 Antibody Pack [NB100-936] - Detection of BRCA1 in U2OS whole cell lysate using NB 100-599. 1 minute ECL detection.](http://images.novusbio.com/fullsize/BRCA1-Antibody-Pack-Western-Blot-NB100-936-img0005.jpg)
![Western Blot: Adipogenesis Antibody Pack [NBP3-20190] - Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/nbp3-20190_adipogenesis-antibody-pack-western-blot-352023131949.jpg "Western Blot: Adipogenesis Antibody Pack [NBP3-20190] - Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Western Blot: Adipogenesis Antibody Pack [NBP3-20190] - Western Blot: CD36 Antibody [NB400-144] - Total protein from Human Skin and Adipose tissue, Mouse Adipose and Rat Adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CD36 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20190_adipogenesis-antibody-pack-western-blot-352023132142.jpg)
![Flow Cytometry: Adipogenesis Antibody Pack [NBP3-20190] - An intracellular stain was performed on U2OS cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/nbp3-20190_adipogenesis-antibody-pack-flow-cytometry-35202313258.jpg)
![Western Blot: Adipogenesis Antibody Pack [NBP3-20190] - Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MBA-MB-468 human breast cancer cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2989) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CREB at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/nbp3-20190_adipogenesis-antibody-pack-western-blot-352023142028..jpg)
![Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742] - Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) by heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 67 kDa (as indicated) using 0.5 ug/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Exosome-Detection-Simple-Western-Antibody-Pack-Simple-Western-NBP3-11742-img0001.jpg "<b>Biological Strategies Validation. </b>Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742] - Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) by heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 67 kDa (as indicated) using 0.5 ug/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.")
![<b>Biological Strategies Validation. </b>Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742] - Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CD63 at approximately 59 kDa (as indicated) using 20 ug/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Exosome-Detection-Simple-Western-Antibody-Pack-Simple-Western-NBP3-11742-img0002.jpg)
![Western Blot: Exosome Detection (Western Blot) Antibody Pack [NBP3-11740] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa) using CD81 Antibody (NB100-65805), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36502), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Exosome-Detection-Western-Blot-Antibody-Pack-Western-Blot-NBP3-11740-img0001.jpg "Western Blot: Exosome Detection (Western Blot) Antibody Pack [NBP3-11740] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa) using CD81 Antibody (NB100-65805), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36502), licensed under a CC-BY license.")
![Western Blot: Exosome Detection (Western Blot) Antibody Pack [NBP3-11740] - Western blot shows lysates of TIME human endothelial cell line and human platelets. PVDF membrane was probed with 2 ug/mL of Mouse Anti-Human CD9 Monoclonal Antibody (Catalog # MAB25292) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD9 at approximately 24 kDa (as indicated).](http://images.novusbio.com/fullsize/Exosome-Detection-Western-Blot-Antibody-Pack-Western-Blot-NBP3-11740-img0003.jpg)
![Exosome Detection (Western Blot) Antibody Pack [NBP3-11740] - Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 ug/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated).](http://images.novusbio.com/fullsize/Exosome-Detection-Western-Blot-Antibody-Pack-NBP3-11740-img0002.jpg)
![Immunohistochemistry-Paraffin: Pancreatic Cell Marker Antibody Pack [NBP3-20186] - Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/nbp3-20186_pancreatic-cell-marker-antibody-pack-immunohistochemistry-paraffin-254202313517.jpg "Immunohistochemistry-Paraffin: Pancreatic Cell Marker Antibody Pack [NBP3-20186] - Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.")
![Immunocytochemistry/Immunofluorescence: Pancreatic Cell Marker Antibody Pack [NBP3-20186] - Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.](http://images.novusbio.com/fullsize/nbp3-20186_pancreatic-cell-marker-antibody-pack-immunocytochemistry-immunofluorescence-352023123430.jpg)
![Immunocytochemistry/Immunofluorescence: Pancreatic Cell Marker Antibody Pack [NBP3-20186] - Immunofluorescence on frozen section of human pancreas using HPx1 Antibody (HIC0-3B3) [NBP1-18951].](http://images.novusbio.com/fullsize/nbp3-20186_pancreatic-cell-marker-antibody-pack-immunocytochemistry-immunofluorescence-352023123932.jpg)
![Western Blot: Pancreatic Cell Marker Antibody Pack [NBP3-20186] - Analysis of Nkx6.1 in human skeletal muscle using NKX6.1 Antibody [NBP1-49672].](http://images.novusbio.com/fullsize/nbp3-20186_pancreatic-cell-marker-antibody-pack-western-blot-35202312443.jpg)
