Pancreatic Cell Marker Antibody Pack

Immunohistochemistry-Paraffin: Pancreatic Cell Marker Antibody Pack [NBP3-20186] - Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry/Immunofluorescence: Pancreatic Cell Marker Antibody Pack [NBP3-20186] - Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry/Immunofluorescence: Pancreatic Cell Marker Antibody Pack [NBP3-20186] - Immunofluorescence on frozen section of human pancreas using HPx1 Antibody (HIC0-3B3) [NBP1-18951].

Western Blot: Pancreatic Cell Marker Antibody Pack [NBP3-20186] - Analysis of Nkx6.1 in human skeletal muscle using NKX6.1 Antibody [NBP1-49672].

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC

Pancreatic Cell Marker Antibody Pack Summary

• Description: This pack contains 1 vial each of: MAB1417-SP (0.025 mg), MAB1249-SP (0.025 mg), NBP1-18951SS (0.025 mL), NBP1-18953SS (0.025 mL), NBP1-18950SS (0.025 mL), NBP1-49672SS (0.025 mL), NL007, NL013 and NL004..
• Immunogen: See individual datasheets

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
• Application Notes: See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.