Siglec-2/CD22 Antibodies

2296-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Siglec-2/ CD22 Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Siglec-2/CD22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2296). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_lightbox"> 2296-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Siglec-2/ CD22 Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Siglec-2/CD22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2296). The ND₅₀ is typically 0.1-0.5 µg/mL." alt="Recombinant Mouse Siglec-2/ CD22 Fc Chimera (Catalog # 2296-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Siglec-2/ CD22 Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Siglec-2/CD22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2296). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF018). A specific band was detected for Siglec‑2/CD22 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Siglec‑2/CD22 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Siglec‑2/CD22 Monoclonal Antibody (Catalog # MAB11515) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Siglec‑2/CD22 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of the germinal centers. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of the germinal centers. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

FAB4867A) and either (A) Mouse Anti-Human Siglec-2/CD22 Monoclonal Antibody (Catalog # MAB1968) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> FAB4867A) and either (A) Mouse Anti-Human Siglec-2/CD22 Monoclonal Antibody (Catalog # MAB1968) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human Siglec-2/CD22 Monoclonal Antibody (Catalog # MAB1968) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

HAF018). A specific band was detected for Siglec-2/CD22 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Siglec-2/CD22 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Siglec-2/CD22 Monoclonal Antibody (Catalog # MAB19681) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Siglec-2/CD22 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for Siglec-2/CD22 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Siglec-2/CD22 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-Siglec-2/CD22 (Y822) Monoclonal Antibody (Catalog # MAB7290) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Siglec-2/CD22 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

AF011), and then Allophycocyanin-conjugated Anti-Goat IgG Tertiary Antibody (F0108). Quadrant markers were set based on secondary plus tertiary antibody staining in the absence of primary antibody. View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> AF011), and then Allophycocyanin-conjugated Anti-Goat IgG Tertiary Antibody (F0108). Quadrant markers were set based on secondary plus tertiary antibody staining in the absence of primary antibody. View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) human Siglec-2/CD22 or (B) irrelevant protein and eGFP was stained with Llama Anti-Human Siglec-2/CD22 Llamabody VHH Monoclonal Antibody (Catalog # LMAB10732) followed by Goat Anti-Llama Secondary Antibody (AF011), and then Allophycocyanin-conjugated Anti-Goat IgG Tertiary Antibody (F0108). Quadrant markers were set based on secondary plus tertiary antibody staining in the absence of primary antibody. View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />