SEC24A Antibodies
We offer SEC24A Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each SEC24A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SEC24A Antibodies can be used in a variety of model species: Human. Use the list below to choose the SEC24A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SEC24A polyclonal antibodies and browse our SEC24A monoclonal antibody catalog.
Alternate Names for SEC24A Antibodies
anti-SEC24A antibody, anti-member A antibody, anti-protein transport protein Sec24A antibody, anti-SEC24 family, member A (S. cerevisiae) antibody, anti-SEC24-related protein A antibody
8 Results for "sec24a" in Primary Antibodies
![Western Blot: SEC24A Antibody (PSH04-36) [NBP3-32936] - Western blot analysis of SEC24A on different lysates with Rabbit anti-SEC24A antibody (NBP3-32936) at 1/2,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HeLa cell lysate Lane 4: U-87 MG cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 24 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32936) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32936_rabbit-sec24a-mab-psh04-36-288202412442955.jpg "Western Blot: SEC24A Antibody (PSH04-36) [NBP3-32936] - Western blot analysis of SEC24A on different lysates with Rabbit anti-SEC24A antibody (NBP3-32936) at 1/2,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HeLa cell lysate Lane 4: U-87 MG cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 24 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32936) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SEC24A Antibody (PSH04-36) [NBP3-32936] - Immunohistochemical analysis of paraffin-embedded human b-cell lymphoma tissue with Rabbit anti-SEC24A antibody (NBP3-32936) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32936) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32936_rabbit-sec24a-mab-psh04-36-298202414262640.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC24A Antibody (PSH04-36) [NBP3-32936] - Immunocytochemistry analysis of Caco-2 cells labeling SEC24A with Rabbit anti-SEC24A antibody (NBP3-32936) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SEC24A antibody (NBP3-32936) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32936_rabbit-sec24a-mab-psh04-36-2920241192313.jpg)
![Flow Cytometry: SEC24A Antibody (PSH04-36) [NBP3-32936] - Flow cytometric analysis of Caco-2 cells labeling SEC24A. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32936, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32936_rabbit-sec24a-mab-psh04-36-29202416372182.jpg)
![Western Blot: SEC24A Antibody (4D9) [H00010802-M01] - The effects of ouabain and low temperature on ER-related chaperones. CFBE/F508del-CFTR cells were treated with 0.1 uM ouabain or 29C (Low-Temp.) for 6, 24, or 48 h, then the cell lysates were analyzed by immunoblotting using the individual antibodies. The bar graph shows quantification of the band intensities for blots expressed as fold change vs. Time 0 h control. Values in the experiments described are presented as means +/- SD (n = 3). Means were tested for statistical significance using a Student's t-test (*P < 0.05). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphar.2012.00176/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SEC24A-Antibody-4D9-Western-Blot-H00010802-M01-img0002.jpg "Western Blot: SEC24A Antibody (4D9) [H00010802-M01] - The effects of ouabain and low temperature on ER-related chaperones. CFBE/F508del-CFTR cells were treated with 0.1 uM ouabain or 29C (Low-Temp.) for 6, 24, or 48 h, then the cell lysates were analyzed by immunoblotting using the individual antibodies. The bar graph shows quantification of the band intensities for blots expressed as fold change vs. Time 0 h control. Values in the experiments described are presented as means +/- SD (n = 3). Means were tested for statistical significance using a Student's t-test (*P < 0.05). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphar.2012.00176/abstract), licensed under a CC-BY license.")
![Sandwich ELISA: SEC24A Antibody (4D9) [H00010802-M01] - Detection limit for recombinant GST tagged SEC24A is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SEC24A-Antibody-4D9-Sandwich-ELISA-H00010802-M01-img0001.jpg)
![Western Blot: SEC24A Antibody (4D9) [H00010802-M01] - The effects of ouabain & low temperature on ER-related chaperones. (A) Left panel: Venn diagram of the intersection between ouabain & low temperature 24 h signatures according to a FDR ≤ 0.05. The numbers indicate distinct genes. The probability *P = 0.0001 was calculated using a hyper-geometric random variable. Right panel: Gene Ontology (GO) cellular processes pie chart of 3530 genes in common between ouabain & low temperature 24 h treatments. (B) The total RNA previously extracted for the microarray analysis was tested by real-time PCR using the individual primers for the gene expressions of HSPA8, HSPA1L, HSPA5, SEC24A, & CANX. Data are presented by the fold change of gene expression vs. DMSO control with mean ± SEM of n = 3 (*P < 0.05) (C) CFBE/F508del-CFTR cells were treated with 0.1 μM ouabain or 29°C (Low-Temp.) for 6, 24, or 48 h, then the cell lysates were analyzed by immunoblotting using the individual antibodies. The bar graph shows quantification of the band intensities for blots expressed as fold change vs. Time 0 h control. Values in the experiments described are presented as means ± SD (n = 3). Means were tested for statistical significance using a Student’s t-test (*P < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23060796), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010802-m01_mouse-monoclonal-sec24a-antibody-4d9-310202415485143.jpg)
![Immunohistochemistry-Paraffin: SEC24A Antibody [NBP3-12265] - Baboon Small Intestine-NBP3-12265. 1:100 dilution in IHC Blocking Buffer. DAB (brown) straining and Hematoxylin QS (blue) counterstrain. 40x magnification on Leica DM000B. FFPE section.](http://images.novusbio.com/fullsize/SEC24A-Antibody-Immunohistochemistry-Paraffin-NBP3-12265-img0001.jpg "Immunohistochemistry-Paraffin: SEC24A Antibody [NBP3-12265] - Baboon Small Intestine-NBP3-12265. 1:100 dilution in IHC Blocking Buffer. DAB (brown) straining and Hematoxylin QS (blue) counterstrain. 40x magnification on Leica DM000B. FFPE section.")
![Western Blot: SEC24A Antibody [NBP2-58626] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/SEC24A-Antibody-Western-Blot-NBP2-58626-img0003.jpg "Western Blot: SEC24A Antibody [NBP2-58626] - Analysis in human cell line U-87 MG.")
![Western Blot: SEC24A Antibody [NBP2-88231] - Host: Rabbit. Target Name: SEC24A. Sample Tissue: Human 786-0 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/SEC24A-Antibody-Western-Blot-NBP2-88231-img0001.jpg "Western Blot: SEC24A Antibody [NBP2-88231] - Host: Rabbit. Target Name: SEC24A. Sample Tissue: Human 786-0 Whole Cell lysates. Antibody Dilution: 1ug/ml")
![Western Blot: SEC24A Antibody [NBP2-88230] - Host: Rabbit. Target Name: SEC24A. Sample Tissue: Human NCI-H226 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/SEC24A-Antibody-Western-Blot-NBP2-88230-img0001.jpg "Western Blot: SEC24A Antibody [NBP2-88230] - Host: Rabbit. Target Name: SEC24A. Sample Tissue: Human NCI-H226 Whole Cell lysates. Antibody Dilution: 1ug/ml")
![Western Blot: SEC24A Antibody [NBP2-88232] - Host: Rabbit. Target Name: SEC24A. Sample Tissue: Human 786-0 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/SEC24A-Antibody-Western-Blot-NBP2-88232-img0001.jpg "Western Blot: SEC24A Antibody [NBP2-88232] - Host: Rabbit. Target Name: SEC24A. Sample Tissue: Human 786-0 Whole Cell lysates. Antibody Dilution: 1ug/ml")