RelA/NFkB p65 Antibodies
We offer RelA/NFkB p65 Antibodies for use in common research applications: Chromatin Immunoprecipitation (ChIP), ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Microarray, Proximity Ligation Assay, Simple Western, Western Blot. Each RelA/NFkB p65 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RelA/NFkB p65 Antibodies can be used in a variety of model species: Human, Mouse, Primate, Rabbit, Rat. Use the list below to choose the RelA/NFkB p65 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RelA/NFkB p65 polyclonal antibodies and browse our RelA/NFkB p65 monoclonal antibody catalog.
Alternate Names for RelA/NFkB p65 Antibodies
anti-RelA/NFkB p65 antibody, anti-RELA antibody, anti-MGC131774 antibody, anti-nf kb p65 antibody, anti-NF-kB p65 antibody, anti-NFkB p65 antibody, anti-NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)) antibody, anti-Nuclear factor NF-kappa-B p65 subunit antibody, anti-Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65 antibody, anti-p65 antibody, anti-rela p65 antibody, anti-v-rel reticuloendotheliosis viral oncogene homolog A (avian) antibody, anti-v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65 antibody
20 Results for "rela-nfkb-p65" in Primary Antibodies
![Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-278202415212453.jpg "Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32648) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29820241435276.jpg)
![Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunocytochemistry analysis of NIH/3T3 cells treated with or without 50μg/mL TNF-alpha for 20 minutes labeling RelA/NFkB p65 with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29202410484811.jpg)
![Flow Cytometry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Flow cytometric analysis of NIH/3T3 cells labeling RelA/NFkB p65. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32648, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-2920241637214.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77827] - Probed against Normal (Lane 1) and TNFalpha (Lane 2) Stimulated HeLa whole cell lysates. A band was observed between 55 and 72 kD corresponding to the expected MW of NFkB p65. The observed higher MW bands have not been characterized.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77827-img0001.jpg "Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77827] - Probed against Normal (Lane 1) and TNFalpha (Lane 2) Stimulated HeLa whole cell lysates. A band was observed between 55 and 72 kD corresponding to the expected MW of NFkB p65. The observed higher MW bands have not been characterized.")
![Immunoprecipitation: RelA/NFkB p65 Antibody [NB100-97831] - Detection of Human RelA/p65 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RelA/p65 antibody NB100-97831 used for IP at 3 mcg/mg lysate. RelA/p65 was also immunoprecipitated by rabbit anti-RelA/p65 antibody NB100-97832, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-Immunoprecipitation-NB100-97831-img0017.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: RelA/NFkB p65 Antibody [NB100-97831] - Detection of Human RelA/p65 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RelA/p65 antibody NB100-97831 used for IP at 3 mcg/mg lysate. RelA/p65 was also immunoprecipitated by rabbit anti-RelA/p65 antibody NB100-97832, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody [NBP2-56067] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56067-img0001.jpg "Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody [NBP2-56067] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.")
