PPP2R5D Antibodies
We offer PPP2R5D Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each PPP2R5D Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PPP2R5D Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PPP2R5D Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PPP2R5D polyclonal antibodies and browse our PPP2R5D monoclonal antibody catalog.
Alternate Names for PPP2R5D Antibodies
anti-PPP2R5D antibody, anti-MGC2134 antibody, anti-MGC8949 antibody, anti-PP2A B subunit isoform B'-delta antibody, anti-PP2A B subunit isoform B56-delta antibody, anti-PP2A B subunit isoform PR61-delta antibody, anti-PP2A B subunit isoform R5-delta antibody, anti-PP2A, B subunit, B' delta isoform antibody, anti-PP2A, B subunit, B56 delta isoform antibody, anti-PP2A, B subunit, PR61 delta isoform antibody, anti-PP2A, B subunit, R5 delta isoform antibody, anti-protein phosphatase 2, regulatory subunit B', delta antibody, anti-regulatory subunit B (B56), delta isoform antibody, anti-regulatory subunit B', delta isoform antibody, anti-Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, deltaisoform antibody, anti-serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
12 Results for "ppp2r5d" in Primary Antibodies
![Western Blot: PPP2R5D Antibody [NB100-846] - Staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer) with antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NB100-846-img0002.jpg "Western Blot: PPP2R5D Antibody [NB100-846] - Staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer) with antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PPP2R5D Antibody [NB100-846] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Immunocytochemistry-Immunofluorescence-NB100-846-img0003.jpg)
![Flow Cytometry: PPP2R5D Antibody [NB100-846] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Flow-Cytometry-NB100-846-img0004.jpg)
![Western Blot: PPP2R5D Antibody [NB100-846] - Staining of A431 (A), Jurkat (B) and HepG2 (C) cell lysate (35 ug protein in RIPA buffer) with antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NB100-846-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PPP2R5D Antibody [NBP1-88959] - Analysis using Anti-PPP2R5D antibody NBP1-88959 (A) shows similar pattern to independent antibody NBP1-88960 (B).](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NBP1-88959-img0009.jpg)
![Immunohistochemistry-Paraffin: PPP2R5D Antibody [NBP1-88959] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Immunohistochemistry-Paraffin-NBP1-88959-img0008.jpg)
![Western Blot: PPP2R5D Antibody [NBP1-88959] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NBP1-88959-img0006.jpg)
![Western Blot: PPP2R5D Antibody (5E7) [NBP2-45387] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D .](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-5E7-Western-Blot-NBP2-45387-img0005.jpg "Western Blot: PPP2R5D Antibody (5E7) [NBP2-45387] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D .")
![Immunohistochemistry: PPP2R5D Antibody (5E7) [NBP2-45387] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-5E7-Immunohistochemistry-NBP2-45387-img0004.jpg)
![Immunohistochemistry: PPP2R5D Antibody (5E7) [NBP2-45387] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-5E7-Immunohistochemistry-NBP2-45387-img0001.jpg)
![Immunohistochemistry: PPP2R5D Antibody (5E7) [NBP2-45387] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-5E7-Immunohistochemistry-NBP2-45387-img0002.jpg)
![Immunohistochemistry: PPP2R5D Antibody (5E7) [NBP2-45387] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-5E7-Immunohistochemistry-NBP2-45387-img0003.jpg)
![Western Blot: PPP2R5D Antibody [NBP1-88960] - Analysis using Anti-PPP2R5D antibody NBP1-88960 (A) shows similar pattern to independent antibody NBP1-88959 (B).](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NBP1-88960-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PPP2R5D Antibody [NBP1-88960] - Analysis using Anti-PPP2R5D antibody NBP1-88960 (A) shows similar pattern to independent antibody NBP1-88959 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PPP2R5D Antibody [NBP1-88960] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Immunohistochemistry-Paraffin-NBP1-88960-img0007.jpg)
![Western Blot: PPP2R5D Antibody [NBP1-88960] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NBP1-88960-img0006.jpg)
![Western Blot: PPP2R5D Antibody (SR2044) [NBP3-22590] - Western blot analysis using NBP3-22590 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22590_rabbit-ppp2r5d-mab-sr2044-57202317215114.jpg "Western Blot: PPP2R5D Antibody (SR2044) [NBP3-22590] - Western blot analysis using NBP3-22590 in HeLa cell lysate.")
![Western Blot: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Human brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32847) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-288202412461614.jpg "Western Blot: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Human brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32847) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32847) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-298202414304124.jpg)
![Flow Cytometry: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Flow cytometric analysis of A431 cells labeling PPP2R5D. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32847, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-392024884664.jpg)
![Western Blot: PPP2R5D Antibody (1A3) [H00005528-M21] - Analysis of PPP2R5D expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-1A3-Western-Blot-H00005528-M21-img0004.jpg "Western Blot: PPP2R5D Antibody (1A3) [H00005528-M21] - Analysis of PPP2R5D expression in Jurkat (Cat # L017V1).")
![Immunocytochemistry/Immunofluorescence: PPP2R5D Antibody (1A3) [H00005528-M21] - Analysis of monoclonal antibody to PPP2R5D on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-1A3-Immunocytochemistry-Immunofluorescence-H00005528-M21-img0002.jpg)
![Sandwich ELISA: PPP2R5D Antibody (1A3) [H00005528-M21] - Detection limit for recombinant GST tagged PPP2R5D is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-1A3-Sandwich-ELISA-H00005528-M21-img0003.jpg)
![Western Blot: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Human brain tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32846) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-288202412442968.jpg "Western Blot: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Human brain tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32846) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Immunocytochemistry analysis of A431 cells labeling PPP2R5D with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-2920241192344.jpg)
![Flow Cytometry: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Flow cytometric analysis of A431 cells labeling PPP2R5D. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32846, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-29202416372164.jpg)
![Western Blot: PPP2R5D Antibody [NB100-57536] - Detection of Human PPP2R5D on HeLa whole cell lysate using NB100-57536. PPP2R5D was also immunoprecipitated by rabbit anti-PPP2R5D antibodies NB100-57537 and NB100-57538.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NB100-57536-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PPP2R5D Antibody [NB100-57536] - Detection of Human PPP2R5D on HeLa whole cell lysate using NB100-57536. PPP2R5D was also immunoprecipitated by rabbit anti-PPP2R5D antibodies NB100-57537 and NB100-57538.")
![Western Blot: PPP2R5D Antibody [NB100-57538] - Detection of Human and Mouse PPP2R5D on HeLa whole cell lysate using NB100-57538. PPP2R5D was also immunoprecipitated by rabbit anti-PPP2R5D antibodies NB100-57536 and NB100-57537.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NB100-57538-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PPP2R5D Antibody [NB100-57538] - Detection of Human and Mouse PPP2R5D on HeLa whole cell lysate using NB100-57538. PPP2R5D was also immunoprecipitated by rabbit anti-PPP2R5D antibodies NB100-57536 and NB100-57537.")
![Western Blot: PPP2R5D Antibody (4E12) [H00005528-M01] - Analysis of PPP2R5D expression in transfected 293T cell line by PPP2R5D monoclonal antibody (M01), clone 4E12.Lane 1: PPP2R5D transfected lysate(70 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-4E12-Western-Blot-H00005528-M01-img0002.jpg "Western Blot: PPP2R5D Antibody (4E12) [H00005528-M01] - Analysis of PPP2R5D expression in transfected 293T cell line by PPP2R5D monoclonal antibody (M01), clone 4E12.Lane 1: PPP2R5D transfected lysate(70 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: PPP2R5D Antibody (4E12) [H00005528-M01] - Detection limit for recombinant GST tagged PPP2R5D is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-4E12-Sandwich-ELISA-H00005528-M01-img0001.jpg)