PBEF/Visfatin/NAMPT Antibodies

HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of 293T human embryonic kidney cell line, RAW 264.7 mouse monocyte/ macrophage cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat PBEF/ Visfatin Monoclonal Antibody (Catalog # MAB40441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF016). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human heart tissue, 293T human embryonic kidney cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human PBEF/Visfatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4335) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

5-001-A, open histogram), followed by Northern-Lights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011) To facilitate intra-cellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> 5-001-A, open histogram), followed by Northern-Lights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011) To facilitate intra-cellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Rat Anti-Human/Mouse PBEF/ Visfatin Monoclonal Antibody (Catalog # MAB4044, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

HAF019). A specific band was detected for PBEF at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF019). A specific band was detected for PBEF at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of H4-II-E-C3 rat hepatoma cell line and rat liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Rat PBEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6384) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PBEF at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

DY990). Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human PBEF/Visfatin protein was serially diluted 2-fold and captured by Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB4335) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

MAB4335) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB4335) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human PBEF/Visfatin protein was serially diluted 2-fold and captured by Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB4335) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />