NT5C Antibodies
We offer NT5C Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot. Each NT5C Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NT5C Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the NT5C Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NT5C polyclonal antibodies.
Alternate Names for NT5C Antibodies
anti-NT5C antibody, anti-3'-pyrimidine nucleotidase antibody, anti-5' nucleotidase, deoxy (pyrimidine), cytosolic type C antibody, anti-5', 3'-nucleotidase, cytosolic antibody, anti-cdN antibody, anti-Cytosolic 5' antibody, anti-Deoxy-5'-nucleotidase 1 antibody, anti-dNT-15'(3')-deoxyribonucleotidase, cytosolic type antibody, anti-DNT1DNT-1 antibody, anti-EC 3.1.3.- antibody, anti-P5N2 antibody, anti-PN-I antibody, anti-PN-II antibody, anti-UMPH2DNT antibody, anti-uridine 5'-monophosphate phosphohydrolase 2 antibody, anti-uridine 5-prime monophosphate hydrolase 2 antibody
6 Results for "nt5c" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: NT5C Antibody [NBP1-84563] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NT5C-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84563-img0011.jpg "Immunocytochemistry/Immunofluorescence: NT5C Antibody [NBP1-84563] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.")
![Simple Western: NT5C Antibody [NBP1-84563] - Simple Western lane view shows a specific band for NT5C in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NT5C-Antibody-Simple-Western-NBP1-84563-img0007.jpg)
![Simple Western: NT5C Antibody [NBP1-84563] - Simple Western lane view shows a specific band for NT5C in 0.2 mg/ml of U-2OS lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NT5C-Antibody-Simple-Western-NBP1-84563-img0008.jpg)
![Simple Western: NT5C Antibody [NBP1-84563] - Electropherogram image(s) of corresponding Simple Western lane view. NT5C antibody was used at 1:20 dilution on U-2OS lysates(s).](http://images.novusbio.com/fullsize/NT5C-Antibody-Simple-Western-NBP1-84563-img0009.jpg)
![Western Blot: NT5C Antibody [NBP1-84563] - Regulation of NT5C S184 phosphorylation.(a) Sensitivity of S184 phosphorylation to PI3K pathway inhibitors. Primary MEFs were treated with A66 (3 μM), MK-2206 (1 μM), Rapamycin (20 nM), U0126 (10 μM) or DMSO as control for 3 h or overnight (o/n) for 16–18 h before lysis. (left) Lysates were immunoblotted as indicated. (right) Quantification of 4 independent experiments. Values are expressed relative to DMSO treated Pik3caH1047R/WT cells. Error bars are sem, **p < 0.01. (b) Sensitivity of S184 phosphorylation to growth factor deprivation. Indicated primary MEFs were grown in 10% FBS or starved overnight in 0.1% FBS. (top) Lysates were immunoblotted as indicated. (bottom) Quantification of 5 independent experiments. Values are expressed relative to Pik3caH1047R/WT cells grown in 10% FBS. Error bars are sem, ***p < 0.001. (c) Sensitivity of S184 phosphorylation to growth factor stimulation. Primary MEFs were starved overnight in 0.1% FBS & stimulated with FBS (10%), Insulin (100 nM) or EGF (100 ng/ml) for the indicated times before lysis. DMSO or MK-2206 (1 μM) was added at same time as stimuli. (top) Lysates were immunoblotted as indicated. (bottom) Quantification of 3–5 independent experiments. Values are expressed relative to time 0 for each stimuli. Error bars are sem. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28059163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84563_rabbit-polyclonal-nt5c-antibody-31020241612237.jpg)
![Western Blot: NT5C Antibody [NBP1-84563] - Impact of S184 phosphorylation of NT5C catalytic activity.(a) S184 phosphorylation does not regulate NT5C nucleotidase activity in vitro. (left) Immunoprecipitates of Flag-NT5C ectopically expressed in HEK293 cells were incubated with 5 mM of the indicated nucleotides. Phosphate release was measured using a malachite green colorimetric assay & expressed as a percent of total nucleotide. The experiment was performed in duplicate & repeated 3 times independently. Error bars are sem. (right) Representative immunoblot from experimental cells. (b) Cells expressing Pik3caH1047R have elevated dNTP levels. (left, middle) Nucleotides were extracted from primary MEFs & analysed by UPLC-MS/MS. The experiment was performed in triplicate & repeated 4 times independently. Error bars are sem, *p < 0.05, **p < 0.01. (right) Representative immunoblot from experimental cells. (c) Effect of NT5C knockdown on cellular dCTP levels. (left) Nucleotides were extracted from primary MEFs, stably expressing indicated siRNA, & analysed by UPLC-MS/MS. The experiment was performed in triplicate & repeated 3 times independently. Error bars are sem. (right) Representative immunoblot from experimental cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28059163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84563_rabbit-polyclonal-nt5c-antibody-310202416121989.jpg)
![Western Blot: NT5C Antibody [NBP1-84563] - Regulation of NT5C S184 phosphorylation.(a) Sensitivity of S184 phosphorylation to PI3K pathway inhibitors. Primary MEFs were treated with A66 (3 μM), MK-2206 (1 μM), Rapamycin (20 nM), U0126 (10 μM) or DMSO as control for 3 h or overnight (o/n) for 16–18 h before lysis. (left) Lysates were immunoblotted as indicated. (right) Quantification of 4 independent experiments. Values are expressed relative to DMSO treated Pik3caH1047R/WT cells. Error bars are sem, **p < 0.01. (b) Sensitivity of S184 phosphorylation to growth factor deprivation. Indicated primary MEFs were grown in 10% FBS or starved overnight in 0.1% FBS. (top) Lysates were immunoblotted as indicated. (bottom) Quantification of 5 independent experiments. Values are expressed relative to Pik3caH1047R/WT cells grown in 10% FBS. Error bars are sem, ***p < 0.001. (c) Sensitivity of S184 phosphorylation to growth factor stimulation. Primary MEFs were starved overnight in 0.1% FBS & stimulated with FBS (10%), Insulin (100 nM) or EGF (100 ng/ml) for the indicated times before lysis. DMSO or MK-2206 (1 μM) was added at same time as stimuli. (top) Lysates were immunoblotted as indicated. (bottom) Quantification of 3–5 independent experiments. Values are expressed relative to time 0 for each stimuli. Error bars are sem. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28059163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84563_rabbit-polyclonal-nt5c-antibody-310202416113523.jpg)
![Western Blot: NT5C Antibody [NBP1-84563] - Impact of S184 phosphorylation of NT5C catalytic activity.(a) S184 phosphorylation does not regulate NT5C nucleotidase activity in vitro. (left) Immunoprecipitates of Flag-NT5C ectopically expressed in HEK293 cells were incubated with 5 mM of the indicated nucleotides. Phosphate release was measured using a malachite green colorimetric assay & expressed as a percent of total nucleotide. The experiment was performed in duplicate & repeated 3 times independently. Error bars are sem. (right) Representative immunoblot from experimental cells. (b) Cells expressing Pik3caH1047R have elevated dNTP levels. (left, middle) Nucleotides were extracted from primary MEFs & analysed by UPLC-MS/MS. The experiment was performed in triplicate & repeated 4 times independently. Error bars are sem, *p < 0.05, **p < 0.01. (right) Representative immunoblot from experimental cells. (c) Effect of NT5C knockdown on cellular dCTP levels. (left) Nucleotides were extracted from primary MEFs, stably expressing indicated siRNA, & analysed by UPLC-MS/MS. The experiment was performed in triplicate & repeated 3 times independently. Error bars are sem. (right) Representative immunoblot from experimental cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28059163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84563_rabbit-polyclonal-nt5c-antibody-31020241612211.jpg)
![Western Blot: NT5C Antibody [NBP1-84563] - Regulation of NT5C S184 phosphorylation.(a) Sensitivity of S184 phosphorylation to PI3K pathway inhibitors. Primary MEFs were treated with A66 (3 μM), MK-2206 (1 μM), Rapamycin (20 nM), U0126 (10 μM) or DMSO as control for 3 h or overnight (o/n) for 16–18 h before lysis. (left) Lysates were immunoblotted as indicated. (right) Quantification of 4 independent experiments. Values are expressed relative to DMSO treated Pik3caH1047R/WT cells. Error bars are sem, **p < 0.01. (b) Sensitivity of S184 phosphorylation to growth factor deprivation. Indicated primary MEFs were grown in 10% FBS or starved overnight in 0.1% FBS. (top) Lysates were immunoblotted as indicated. (bottom) Quantification of 5 independent experiments. Values are expressed relative to Pik3caH1047R/WT cells grown in 10% FBS. Error bars are sem, ***p < 0.001. (c) Sensitivity of S184 phosphorylation to growth factor stimulation. Primary MEFs were starved overnight in 0.1% FBS & stimulated with FBS (10%), Insulin (100 nM) or EGF (100 ng/ml) for the indicated times before lysis. DMSO or MK-2206 (1 μM) was added at same time as stimuli. (top) Lysates were immunoblotted as indicated. (bottom) Quantification of 3–5 independent experiments. Values are expressed relative to time 0 for each stimuli. Error bars are sem. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28059163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84563_rabbit-polyclonal-nt5c-antibody-310202416114732.jpg)
![Immunohistochemistry-Paraffin: NT5C Antibody [NBP1-84563] -Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/antibody/nbp1-84563_rabbit-polyclonal-nt5c-antibody-immunohistochemistry-paraffin-1312202495154..png)
![Immunohistochemistry-Paraffin: NT5C Antibody [NBP1-84563] -Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/antibody/nbp1-84563_rabbit-polyclonal-nt5c-antibody-immunohistochemistry-paraffin-1312202495245..png)
![Immunohistochemistry-Paraffin: NT5C Antibody [NBP1-84563] -Staining of human liver shows weak cytoplasmic positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-84563_rabbit-polyclonal-nt5c-antibody-immunohistochemistry-paraffin-1312202495335..png)
![Western Blot: NT5C Antibody [H00030833-B01P] - Analysis of NT5C expression in transfected 293T cell line by NT5C polyclonal antibody. Lane 1: NT5C transfected lysate(12.87 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NT5C-Antibody-Western-Blot-H00030833-B01P-img0006.jpg "Western Blot: NT5C Antibody [H00030833-B01P] - Analysis of NT5C expression in transfected 293T cell line by NT5C polyclonal antibody. Lane 1: NT5C transfected lysate(12.87 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NT5C Antibody [H00030833-B01P] - Analysis of purified antibody to NT5C on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/NT5C-Antibody-Immunocytochemistry-Immunofluorescence-H00030833-B01P-img0004.jpg)
![Immunohistochemistry-Paraffin: NT5C Antibody [H00030833-B01P] - Analysis of purified antibody to NT5C on formalin-fixed paraffin-embedded human lung. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/NT5C-Antibody-Immunohistochemistry-Paraffin-H00030833-B01P-img0005.jpg)
![Immunohistochemistry-Paraffin: NT5C Antibody [NBP1-84564] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/NT5C-Antibody-Immunohistochemistry-Paraffin-NBP1-84564-img0008.jpg "Immunohistochemistry-Paraffin: NT5C Antibody [NBP1-84564] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.")
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-84564_rabbit-polyclonal-nt5c-antibody-104202515421467.jpg)
![Immunohistochemistry: NT5C Antibody - BSA Free [NBP3-47301] - Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using (NT5C Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-47301_rabbit-nt5c-pab-bsa-free-284202512283365.jpg "Immunohistochemistry: NT5C Antibody - BSA Free [NBP3-47301] - Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using (NT5C Antibody) at dilution of 1:50")
![Western Blot: NT5C Antibody [NBP2-19596] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NT5C-Antibody-Western-Blot-NBP2-19596-img0001.jpg "Western Blot: NT5C Antibody [NBP2-19596] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: NT5C Antibody [H00030833-D01P] - Analysis of NT5C expression in transfected 293T cell line by NT5C polyclonal antibody.Lane 1: NT5C transfected lysate(13.30 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NT5C-Antibody-Western-Blot-H00030833-D01P-img0001.jpg "Western Blot: NT5C Antibody [H00030833-D01P] - Analysis of NT5C expression in transfected 293T cell line by NT5C polyclonal antibody.Lane 1: NT5C transfected lysate(13.30 KDa).Lane 2: Non-transfected lysate.")