IL-17/IL-17A Antibodies

FAB554P) and either (A) Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Catalog # MAB7211) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)." class="big_lightbox"> FAB554P) and either (A) Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Catalog # MAB7211) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)." alt="Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Catalog # MAB7211) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)." class="big_thumb" />

5848-CL) induces IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). IL-6 Secretion elicited by Recombinant Canine IL-17 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-17 Monoclonal Antibody (Catalog # MAB5848). The ND₅₀ is typically 5-30 ng/mL." class="big_lightbox"> 5848-CL) induces IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). IL-6 Secretion elicited by Recombinant Canine IL-17 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-17 Monoclonal Antibody (Catalog # MAB5848). The ND₅₀ is typically 5-30 ng/mL." alt="Recombinant Canine IL-17 (Catalog # 5848-CL) induces IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). IL-6 Secretion elicited by Recombinant Canine IL-17 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-17 Monoclonal Antibody (Catalog # MAB5848). The ND₅₀ is typically 5-30 ng/mL." class="big_thumb" />

5837-IL), the the presence of Recombinant Mouse TNF-alpha (0.5 ng/mL, 410-MT), stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (M6000B). IL-6 secretion elicited by Recombinant Human IL-17A/F Heterodimer (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-17A Monoclonal Antibody (Catalog # MAB31711). The ND₅₀ is typically 1-6 μg/mL." class="big_lightbox"> 5837-IL), the the presence of Recombinant Mouse TNF-alpha (0.5 ng/mL, 410-MT), stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (M6000B). IL-6 secretion elicited by Recombinant Human IL-17A/F Heterodimer (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-17A Monoclonal Antibody (Catalog # MAB31711). The ND₅₀ is typically 1-6 μg/mL." alt="Recombinant Human IL-17A/F Heterodimer (5837-IL), the the presence of Recombinant Mouse TNF-alpha (0.5 ng/mL, 410-MT), stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (M6000B). IL-6 secretion elicited by Recombinant Human IL-17A/F Heterodimer (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-17A Monoclonal Antibody (Catalog # MAB31711). The ND₅₀ is typically 1-6 μg/mL." class="big_thumb" />